摘要
目的观察深海鳕鱼胶原蛋白肽敷料并维生素E对大鼠皮肤创面愈合速度与愈后瘢痕影响。方法成年Wistar大鼠45只,随机分为对照组(A组)、胶原蛋白组(B组)和胶原蛋白+维生素E组(C组)3组。大鼠背部制作全层皮肤伤口模型后,每日A组外敷蒸馏水,B组外敷蒸馏水和深海鳕鱼胶原蛋白肽敷料,C组外敷蒸馏水、深海鳕鱼胶原蛋白肽和维生素E复合敷料。计算各组大鼠治疗过程中创面愈合率,并于伤后第11天取伤口组织进行免疫组织化学染色,观察伤口组织细胞中细胞角蛋白19(CK19)、β1整合素、抗肿瘤蛋白63α(△NP63α)、Ⅰ型胶原和Ⅲ型胶原的表达。结果 3组大鼠创面愈合率比较,差异有显著性(F=18.84,P<0.05),且各组间比较差异均有显著性(t=2.32~6.08,P<0.05)。伤后第11天,3组大鼠创面细胞中CK19、β1整合素、Ⅰ型胶原和Ⅲ型胶原表达量比较,差异有显著性(F=4.64~21.57,P<0.05)。结论深海鳕鱼胶原蛋白肽敷料并维生素E可促进大鼠全层皮肤伤口愈合并减少瘢痕形成。
Objective To investigate the effect of deep-sea cod collagen peptide dressing combined with vitamin E on skin wound healing rate and scars in rats. Methods A total of 45 adult Wistar rats were randomly divided into control group（group A）,collagen group（group B）,and collage-vitamin E group（group C）.After a full-thickness skin wound model was established on the back of the rats,the rats in group A were given external application of distilled water,those in group B were given external application of distilled water combined with deep-sea cod collagen peptide dressing,and those in group C were given external application of distilled water combined with deep-sea cod collagen peptide and vitamin E dressing.The wound healing rate was calculated for all groups.Wound tissue samples were collected on day 11 after trauma,and immunohistochemistry was used to observe the expression of cytokeratin 19（CK19）,β1 integrin,anti-tumour protein 63α（△NP63α）,collagen type Ⅰ,and collagen type Ⅲin wound tissue cells. Results There was a significant difference in wound healing rate between the three groups（F=18.84,P0.05）and there were also significant differences between any two groups（t=2.32-6.08,P0.05）.On day 11 after trauma,there were significant differences in the expression of CK19,β1 integrin,collagen typeⅠ,and collagen typeⅢin wound tissue cells between the three groups（F=4.64-21.57,P0.05）. Conclusion Deep-sea cod collagen peptide dressing combined with vitamin E can promote the healing of full-thickness skin wound and reduce the formation of scars in rats.
出处
《青岛大学医学院学报》
CAS
2017年第4期392-395,共4页
Acta Academiae Medicinae Qingdao Universitatis
作者简介
赵芬（1995-），女。
[通讯作者]夏玉军（1962-），男，教授，硕士生导师。
