摘要
目的采用~1H-NMR代谢组学方法,研究苦参碱抑制人肝癌SMMC-7721细胞增殖的作用。方法应用CCK8法检测苦参碱抑制SMMC-7721细胞增殖的作用,并对细胞裂解液及细胞培养上清液进行~1H-NMR检测,结合多元统计分析和代谢通路分析探讨其作用机制。结果 1、2、4 mg/m L苦参碱能显著抑制SMMC-7721细胞的增殖。与对照组相比,苦参碱作用于SMMC-7721细胞后,细胞内外共发现21种差异代谢物。苦参碱能显著调节细胞内外亮氨酸、缬氨酸、甘氨酸等差异代谢物的量,对SMMC-7721细胞的氨基酸代谢起到一定的调控作用。结论苦参碱可能通过调控氨基酸代谢、能量代谢等途径发挥抗肝癌作用。本研究从代谢组学角度为阐释苦参碱抗肝癌的作用机制提供了科学依据。
Objective To investigate the proliferation inhibitory effect of matrine in SMMC-7721 cells using ^1H-NMR metabolomics approach. Methods CCK8 method was used to detect the proliferation inhibitory effect of matrine in SMMC-7721 cells, and the cell lysates and cell culture supernatants were detected by ^1H-NMR combined with multivariate statistical analysis and metabolic pathway analysis with the aim of exploring its mechanism of action. Results Matrine at the concentration of 1, 2, and 4 mg/mL could significantly inhibit the proliferation of SMMC-7721 cells. Compared with control group, 21 differential metabolites were confirmed inside and outside the cells after treatment of matrine. Matrine could significantly regulate the contents of differential metabolites such as leucine, valine and glycine inside and outside the cells, and regulate the amino acid metabolism of hepatoma cells. Conclusion Matrine plays anti-hepatoma effect likely through regulation of amino acid metabolism and energy metabolism. This study provides a scientific basis for elucidation of the anti-hepatoma mechanism of matrine from the perspective of metabolomics.
作者
王珂欣
高丽
周玉枝
秦雪梅
杜冠华
WANG Ke-xin GAO Li ZHOU Yu-zhi QIN Xue-mei DU Guan-hua(Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China Institute ofMateria Medica, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050, China)
出处
《中草药》
CAS
CSCD
北大核心
2017年第20期4275-4283,共9页
Chinese Traditional and Herbal Drugs
基金
山西省研究生联合培养基地人才项目(2016JD05)
山西大学引进人才事业发展经费(226545008
226545003)
山西省科技创新重点团队(201605D131045-18)
地产中药功效物质研究与利用山西省重点实验室(201605D111004)
关键词
苦参碱
肝癌
细胞增殖
代谢组学
氨基酸代谢
能量代谢
matrine
hepatoma
cell proliferation
metabolomics
amino acid metabolism
energy metabolism
作者简介
王珂欣（1992-），女，黑龙江人，研究生在读，药物化学专业，主要从事中药药理学研究。E—mail：nmgwkx1992@163．com
通信作者高丽Tel：（0351）7018379E—mail：gaoli87@sxu．edu.cn
秦雪梅Tel：（0351）7011501E—mail：qinxm@sxu．edu．cn
