摘要
piRNA是继siRNA和miRNA之后发现的一类新型非编码小RNA,其与Piwi蛋白协同作用参与piRNA通路,保护动物生殖细胞的遗传信息免受分子寄生虫如转座子的有害影响。近年来,人们对piRNA发生过程的相关蛋白及生物学作用做了研究,发现多种Piwi亚家族蛋白能与piRNA结合形成Piwi-piRNA复合体,通过表观遗传调控参与生殖细胞形成与发育、生殖干细胞分化、性别决定等生物学功能。本文主要围绕piRNA的形成及特点、Piwi-piRNA复合体发挥生物学作用的方式、piRNA在雌性动物中的生物学功能这3个方面对近期的研究进展进行综述,为深入探究piRNA调控雌性动物繁殖机制提供参考。
Following the discovery of siRNA and miRNA, a new class of small noncoding RNA, called piRNA, was found, piRNA can interact with Piwi subfamily proteins and they involve in piRNA pathways,which can protect genetic information of animal germ cells from the harmful effects of molecular parasites such as transposon. In recent years,piRNA biogenesis-related pro- teins and their biological effects were studied. It was found that a variety of Piwi subfamily proteins could bind to piRNA to form Piwi-piRNA complex. These complexes were involved in germ cells formation and development, germline stem ceils differentiation, sex determination and other biological functions through the way of epigenetic regulation. In the present paper,we reviewed the research progress on the biogenesis and characteristics of piRNA,the ways of Piwi-piRNA complexes playing the biological roles and biological functions of piRNA in female animals, which will provide a reference for further research on regulatory mechanism of piRNA in female animal reproduction.
作者
李春艳
任春环
刘秋月
胡文萍
王翔宇
曹晓涵
张子军
狄冉
储明星
LI Chun-yan REN Chun-huan LIU Qiu-yue HU Wen-ping WANG Xiang-yu CAO Xiao-han ZHANG Zi-jun DI Ran CHU Ming-xing(Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2017年第10期1785-1795,共11页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金项目(31572371
31472078
31402041)
宁夏农林科学院科技创新先导资金项目(DWJLC-2016001)
中国农业科学院科技创新工程(ASTIP-IAS13)
国家肉羊产业技术体系专项(CARS-39)
中央级公益性科研院所基本科研业务费专项(2013ywf-zd-1
2015ywf-zd-2
2015ywf-zd-8)
内蒙古自治区科技重大专项
内蒙古自治区战略性新兴产业发展专项资金计划
作者简介
李春艳（1992-），女，甘肃通渭人，硕士生，主要从事动物分子育种研究，E-mail：3010992163@qq．com
通信作者：狄冉，博士，副研究员，E-mail：dirangirl@163．com；
储明星，博士，研究员，主要从事羊优异繁殖性状的分子机理研究，E—mail：mxchu@263．net
