摘要
本文建立了尿中去氢甲基睾丸素及其代谢物的HPLC分析方法。尿样以固相法在XAD-2树脂柱上提取纯化后再经HPLC分析。检测波长为254 nm,流动相为甲醇-水(100:40V/V)。当尿中药物浓度在0.05～1.0μg/ml时,方法线性关系良好,相关系数为0.9993。本法检测限为5 ng,灵敏度为25ng/ml,日内、日间误差均小于5%。
A simple and sensitive method for the determination of methandienone and its metabolite in human urine by HPLC, using methyotestosterone as internal standard, was developed. The urine sample was first purified by solid phase extraction method, and then determined by HPLC with detective wavelength at 254 nm. The mobile phase was methanol-water (100: 40 V/V). It showed a good linearity between the range of 0.05～1.0 μg/ml with the correlation coefficient of 0.9993. The detective limit was 5 ng, and the sensitivity was 25 ng/ml. The precisions of both within-day and day-to-day were less than 5%. This method was found to be much simpler and more inexpensive than that of GC-MS, and it could serve as an alternative method for the doping control in athletes training.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1991年第2期83-85,共3页
Journal of China Pharmaceutical University
