摘要
目的:体外建立仿生矿化模型,研究氟离子作用下不同浓度明胶基质对矿化过程的影响。方法:将浓度为30 mmol/l钙离子溶液、浓度为5 mmol/l磷酸根溶液和不同浓度明胶溶液分别加入反应装置,反应7 d和21 d后检测阳离子选择膜表面矿化物的形态和化学成分。结果:随反应时间从7 d延长至21 d,离子选择膜表面矿化物的Ca/P从对照组1.39,10%明胶组1.48,20%明胶组1.55,依次变为对照组1.38,10%明胶组1.49,20%明胶组1.67。加入明胶后,晶体形态从无定形片状转变为针状。随明胶浓度升高,晶体之间相互融合,矿化物Ca/P升高。通过X线衍射证实,Ca/P为1.67的产物为羟基磷灰石。结论:明胶能促进仿生矿化体系中晶体合成,使其向羟基磷灰石转变。
Objective： To set up a model and study the effect of gelatin,as organic matrix,on the process of biomineralization.Method： 30 mmol calcium solution and 5 mmol phosphate solution were added into the mineralization device respectively. Gelatin was injected into the reaction space between the cation-exchange membrane and dialysis membrane. The cation-exchange membranes were gained after 7 and 21 days and the sediments on the membranes were prepared for SEM,XRD and EDX. Results： Crystals in the sediment become aciform or prismatical as the reaction time being prolonged. When 10% gelatin was added into the reaction space,the crystals became fasciculate. And the Ca/P proportion of the crystals raised when the gelatin’s concentration was changed to 20%,which meant the transformation of amorphous calcium phosphate to hydroxyapatite. Dentin-like sediments were formed on the ion-selective membrane with 20% gelatin added into the reaction space after 21 days. The crystals appeared typical hexagonal prism or hexagonal cone of around 100 nm in diameter and 2 um in height as they grew along c-axis direction. Analysis by energy dispersive x-ray diffraction showed that these precipitates were HA. Conclusions： Gelatin can promote the process of biomineralization and the transformation from amorphous calcium phosphate to hydroxyapatite.
出处
《口腔生物医学》
2017年第3期150-154,共5页
Oral Biomedicine
基金
国家自然科学基金资助项目(81671033)
关键词
明胶
仿生矿化
阳离子选择膜
gelatin
biomineralization
cation-exchange membranes
作者简介
通信作者：孙宏晨Tel：（0431）88796012 Email：hcsun@jlu.edu.cn
