摘要
为确定桃树细胞质抗寒相关基因,依据模式植物拟南芥中抗寒相关的RBD1基因,结合桃基因组数据设计并克隆到一个桃PpRBD1基因。该基因全长2 230 bp,编码513个氨基酸;结构域分析显示,该氨基酸序列含有RRM(RNA recognition motif)保守结构域,是与拟南芥RBD1同源的蛋白;亚细胞定位预测表明,该基因编码的蛋白可能在叶绿体中表达。该基因可能在桃抵抗寒冷胁迫中发挥重要作用。
The objective of this study was to clone a novel chloroplast gene of PpRBD1 in peach （Prunus persica） according to Arabidopsis thaliana and peach genome data, and to analyze its correlation with cold tolerance. The full length of PpRBD1 gene was 2 230 bp sequence, which encoded a deduced protein including 513 amino acid residues. The domain analysis revealed that the amino acid sequence contained a conserved RRM （RNA recognition motif） domain, which was a protein homologous to RBD1 in Arabidopsis thaliana. Subcellular localization showed that it expressed possible in chloroplast. And it might play an important role in plant tolerance.
出处
《河北科技师范学院学报》
CAS
2017年第2期12-17,共6页
Journal of Hebei Normal University of Science & Technology
基金
国家自然科学基金资助项目(项目编号:31572093)
关键词
桃
抗寒性
叶绿体
ppRBD1
RRM
peach （Prunus persica）
cold tolerance
chloroplast
PpRBD1
RRM
作者简介
张雪钰（1992-），女，硕士研究生。主要研究方向：果树遗传育种与分子生物学。
通讯作者，男，教授，硕士，硕士研究生导师。主要研究方向：桃遗传育种与分子生物学。E—mail：zhanglibin9364@163．com。
