摘要
葡萄是世界上最重要的经济果树之一，据联合国粮食及农业组织统计，截止到2013年，中国葡萄栽培面积已达715．5万hm^2。葡萄总产量超2300万kg。长期以来，葡萄病害是影响葡萄产业健康发展的重要因素。近年来研究发现由葡萄座腔菌科真菌（Botryosphaeriaceae）引起的葡萄溃疡病（Botryosphaeria dieback）是葡萄上的主要枝干病害，病原菌主要通过自然孔口或修剪伤口侵入，感病植株出现枝干溃疡、树势减弱、果梗干枯、果实干缩或掉粒等症状，更严重的造成整个植株枯死。
The occurrence of grapevine canker caused by pathogens of fungal family Botryosphaeriaceae is increasing in most grapevine growing areas worldwide. However, the mechanism of Botryosphaeriaceae-grapevine interaction is still in its infancy. In order to identify Botryosphaeriaceae resistance-related genes and targets of fungal effectors in grapevine, a yeast two-hybrid cDNA library of tobacco infected by Lasiodiplodia theobromae CSS-01s was constructed by SMART cDNA synthesis technology. The results showed that the titer of cDNA library was 1.2×10^8 CFU·mL^-1. Results of PCR amplification of 24 clones randomly selected from the cDNA library revealed that the length of inserted cDNA fragments ranged from 350 bp to 2 000 bp, with a recombination rate of 100%. In addition, the average insert size of the cDNA library was 750 bp, indicating that this library is of high quality and could be applied for subsequent research.
出处
《植物病理学报》
CAS
CSCD
北大核心
2017年第5期716-720,共5页
Acta Phytopathologica Sinica
基金
国家葡萄产业技术体系(CARS-30)
北京市博士后工作经费资助项目~~
作者简介
邢启凯，博士后，主要从事植物病原真菌与寄主的分子互作研究；E—mail：qikaixing@163．com。
通讯作者：李兴红，研究员，主要从事植物真菌病害研究，E-mail：lixinghong1962@163．com
