摘要
目的为了解米非司酮对子宫内膜细胞的毒性,探讨使用米非司酮后子宫内膜细胞凋亡情况以及促血管生成因子水平变化情况。方法取培养的人子宫内膜细胞,观察不同浓度米非司酮的细胞毒性情况,以染色流式细胞术检查细胞凋亡的全科,同时使用实时荧光定量聚合酶链反应观察米非司酮对促血管生成因子水平的影响。结果发现随着药物浓度上升,细胞死亡率增加、细胞解体、排列紊乱现象严重;米非司酮对基因pro-Caspase3表达有抑制作用,药物对Ang-1表达有抑制效果,对VEGF、Ang-2表达有促进效果。结论米非司酮可能通过调控pro-Caspase3表达促进人子宫内膜细胞凋亡有效果,同时此药抑制机体Ang-1表达、增加VEGF、Ang-2表达。
Objective To study the clinical toxicity of Mifepristone and explore its clinical impact in the endometrial cell apoptosis and angiogenesis expression. Methods The endometrial cell was collected and cultured. Based on MTS methods,the cytotoxicity with different concentrations of Mifepristone was detected.Through Western Blot and Annexin V-FITC/PI dyeing flow cytometry,the influence of Mifepristone in the endometrial cell apoptosis was observed.With the real-time fluorescence quantitative polymerase chain reaction,the influence of Mifepristone in the angiogenesis expression was observed. Results With the higher drug concentrations,the cell death,cytoclasis and disordered arrangement were more serious.With the higher drug concentrations,the cell viability was increased,and the service time was shortened.Mifepristone restricted the pro-Caspase3 and Ang-1 expression and increased the VEGF and Ang-2 expression. Conclusion Through the regulation of pro-Caspase3 expression,Mifepristone accelerates the endometrial cell apoptosis.Mifepristone restricts the Ang-1 expression and increases the VEGF and Ang-2 expression.
出处
《中国医药科学》
2017年第17期33-35,53,共4页
China Medicine And Pharmacy
