摘要
目的通过检测S100B蛋白、脑源性神经营养因子(BDNF)、胰岛素样生长因子-1(IGF-1)在癫痫婴儿血清中的水平,探讨其与婴儿癫痫发作的相关性。方法选取2012年1月至2016年12月收治的88例发作性疾病婴儿为研究对象,按发作类型分为48例癫痫发作组及40例非癫痫性发作组。酶联免疫吸附测定(ELISA)法测定婴儿发作性表现后4、24 h血清S100B蛋白、BDNF、IGF-1水平。另外选择40例同期体检健康婴儿作为对照。比较三组对象及癫痫发作患儿发作后不同时间(4、24 h)、不同发作持续时间(抽搐时间>5 min 21例和抽搐时间≤5 min 27例)、不同发作类型(全面性发作22例,局限性发作26例)血清S100B蛋白、BDNF、IGF-1水平的差异,分析其与癫痫发作的相关性。结果发作后24 h血清S100B蛋白、BDNF、IGF-1水平比较,呈癫痫发作组>非癫痫发作组>对照组(P均<0.01);癫痫组患儿发作后24 h血清三指标水平明显低于发作后4 h(P均<0.01)。抽搐时间>5 min患儿血清S100B蛋白、BDNF、IGF-1水平均高于抽搐时间≤5 min患儿(P均<0.01);血清S100B蛋白水平全面性发作患儿明显高于局限性发作患儿(P<0.01),血清BDNF、IGF-1水平全面性发作与局限性发作患儿比较差异无统计学意义(P均>0.05)。结论血清S100B蛋白、BDNF、IGF-1水平检测有助于癫痫发作的临床诊断,可作为癫痫患儿神经损伤程度评估的参考指标。
Objective To detect the levels of serum S100B protein, brain derived neurotrophic factor （BDNF） and insulin-like growth factor-1 （ IGF-1 ） and explore their associations with infantile seizures. Methods A total of 88 infants with paroxysmal diseases admitted from January 2012 to December 2016 were selected as research objects and were divided into epileptic seizure group （ n = 48） and 40 non-epileptic seizure group （ n = 40） according to seizure type. Enzyme-linked immunosorbent assay （ELISA） method was used to detect serum S100B protein, BDNF and IGF-1 levels 4-,and 24- hours after seizure in paroxysmal diseases infants. Forty heahhy infants were selected as control group. The levels of serum S100B protein, BDNF and IGF-1 in three groups and in epileptic seizure infants with deferent time after seizure （4 and 24 hours）, different seizure duration （convulsionary time 〉 5 min, convulsionary time ≤〈5 min）and different seizure type （generalized seizure, focal seizure） were compared, and their associations with epileptic seizure were analyzed. Results The levels of serum SI00B protein, BDNF and IGF-1 at 24 hours after seizure of paroxysmal diseases progressively decreased in the order of epileptic seizure group, non-epileptic seizure group, control group, and there were significant differences between any two groups（ all P 〈0.01 ）. Levels ,of the three serum indexes at 24 hours after seizure were significantly lower than those at 4 hours after seizure in epileptic seizure group （ all P 〈 0. 01 ） and were significantly higher in epilepsy infants with convulsionary time more than 5 minutes than those in epilepsy infants with convulsionary time less than or equal to 5 minutes （ all P 〈 0.01 ）. Level of serum S100B protein in epilepsy infants with generalized seizure was significantly higher than that in epilepsy infants with focal seizure （ P 〈 0.01 ）. There were no significant differences in levels of serum BDNF and IGF-1 between generalized seizure and focal seizure （ all P 〉 0.05 ）. Conclusion The levels of serum S100B protein, BDNF and IGF-1 contribute to the clinical diagnosis of epileptic seizure and can serve as reference indexes for the evaluation of nerve injury degree in infants with epilepsy.
作者
郝伟红
姜红
马慧平
HAO Wei-hong JIANG Hong MA Hui-ping(Department of Pediatrics, Handan Central Hospital, Handan, Hebei 056001, China)
出处
《中国临床研究》
CAS
2017年第9期1186-1188,1191,共4页
Chinese Journal of Clinical Research
基金
河北省卫生厅科研基金项目(2015045B)
作者简介
通讯作者：姜红，E-mail：mksddf123a@163．com
