摘要
对分离到的一株疑似犬种布鲁氏菌进行生物特性和基因型鉴定,并用宿主实验动物(比格犬)测定了最小感染量和脾含菌量。形态与染色特性、培养特性、血清学特性、生化特性鉴定以及Multi-PCR和16S rRNA测序结果表明,该分离株为犬种布鲁氏菌,其对比格犬的最小感染剂量为10~5 CFU/m L,对应的脾含菌量为4×10~5~3×10~6 CFU/g。
In this study, an isolated strain of Brucella was identified by biological feature and genotype, and determined the minimum infected dose （MID） and bacteria content of spleen in experimental animals （beagle）. This isolated strain is Brucella cains which was identified by dyeing properties, culture characteristics, serological characteristics, biochemical characteristics, and further identified by Muhi-PCR and 16S rRNA. The MID is 105 CFU/mL in beagle, and the content is 4×105- 3× 106 CFU/g in spleen.
出处
《中国兽药杂志》
北大核心
2017年第9期1-6,共6页
Chinese Journal of Veterinary Drug
关键词
犬种布鲁氏菌
分离鉴定
最小感染剂量
脾含菌量
Brucella canis
isolation and infection
minimum infected dose （MID）
the content of becteria in spleen
作者简介
张贺，硕士研究生，从事布鲁氏菌病疫苗研究。
通讯作者：毛开荣，E-mail：kairongmao@sohu．com；
周双海，E-mail：13522630832@163．com
