摘要
[目的]探讨高温联合紫外线2~4个月连续暴露对大鼠血清白介素-21(IL-21)水平的影响。[方法]选取体重为(200±10)g的健康成年SD大鼠112只,雌雄各半。随机分为对照组、高温组、紫外线组以及联合组(高温+紫外线),每组雌雄各14只,分笼饲养。在紫外灯(310 nm,72μW/cm2)和(38±1)℃高温环境中每天暴露2 h,连续暴露2个月和4个月后分别处死,采用酶联免疫吸附试验对大鼠血清IL-21进行检测。[结果]高温联合紫外线分别暴露2个月及4个月后,联合组雄性大鼠最终体重[(428.57±12.94)、(476.86±13.14)g],体重增长量[(183.29±11.74)、(306.86±13.81)g]明显低于对照组[最终体重:(493.57±17.12)、(587.00±11.53)g;体重增长量:(249.00±17.99)、(414.43±12.41)g](均P<0.05)。各组雌性大鼠体重差异均无统计学意义(P>0.05)。暴露2个月后,各暴露组无论雄性、雌性大鼠的脾脏系数和胸腺系数均无差异(P>0.05)。暴露4个月后,各暴露组雄性大鼠脾脏系数[高温组:(1.311±0.037)‰;紫外线组:(1.270±0.058)‰;联合组:(1.197±0.024)‰]均小于对照组[(1.410±0.026)‰](P<0.05);高温组雄性大鼠胸腺系数[(0.576±0.041)‰]小于对照组[(0.996±0.067)‰](P<0.05);而雌性大鼠的脏器系数均无差异(P>0.05)。相同暴露时间和暴露因素,雌、雄大鼠间血清IL-21水平差异均无统计学意义(P>0.05),故将雌雄大鼠合并分析。除2个月紫外线组[(487.69±15.21)ng/L]外,其他各暴露组[2个月:高温组(365.05±18.17)ng/L、联合组(278.66±11.70)ng/L;4个月:高温组(343.73±22.97)ng/L、紫外线组(264.45±23.96)ng/L、联合组(150.90±23.06)ng/L]大鼠血清IL-21水平均低于对照组[2个月(518.11±18.68)ng/L、4个月(582.18±28.90)ng/L](P<0.05);紫外线组和联合组大鼠暴露4个月时血清IL-21水平均低于暴露2个月时(P<0.01),无论暴露2个月还是4个月,联合组大鼠血清IL-21水平均明显低于高温组和紫外线组(P<0.05)。[结论]高温联合紫外线暴露对大鼠体重增长量、免疫器官重量以及血清IL-21水平均有一定的影响,且随着暴露时间的延长,血清IL-21含量呈降低趋势。
A total of 112 healthy adult SI） rats, （200 ± 10）g, half male and half female, were randomly divided into control group, high temperature group, ultraviolet group, and combined group （high temperature＋ultraviolet）, with 14 female and 14 male rats in each group and raised separately. The rats were correspondingly exposed to high temperature [（38 ± 1）℃ ] and ultraviolet （310nm, 72 μW/cm^2） for 2h per day. After continuous exposure for 2 or 4 months, the rats were sacrificed and serum IL-21 was measured by ELISA. [ Results ] After being exposed to high temperature combined with ultraviolet for 2 months and 4 months, the final weight [（428.57 ± 12.94）, （476.86 ± 13.14）g] and weight gain [（183.29 ± 11.74）, （306.86 ± 13.81）g] of male SD rats were significantly lower than those of the control group [final weight： （493.57 ± 17.12）, （587.00 ± 11.53）g; weight gain： （249.00 ± 17.99）, （414.43 ± 12.41）g] （all Ps 〈 0.05）, respectively. No significant differences in body weight were found for female rats （P 〉 0.05）. After continuous exposure for 2 months, no statistical difference was found in the spleen coefficients and thymus coefficients by all exposure treatments and by gender （P〉 0.05）. After continuous exposure for 4 months, the spleen coefficients of each exposed group [high temperature group： （ 1.311 ± 0.037）‰ ; ultraviolet group： （1.270 ± 0.058）‰ ; combined group： （1.197 ± 0.024）‰ ] were smaller than those of the control group [（1.410 ± 0.026）‰ ] （P〈0.05）; the thymus coefficient of male rats in the high temperature group [（0.576 ± 0.041）‰ ] was lower than that of the control group [（0.996 ± 0.067）‰ ] （P 〈 0.05）; there was no difference in organ coefficients for the female rats administrated with different exposures （P〉0.05）. Under the same exposure time and exposure treatments, there was no gender difference in serum IL-21 levels （P 〉 0.05）; therefore, the female and male rats were merged for further analysis. Except the ultraviolet group after 2 months [（487.69 ±15.21）ng/L], the levels of serum IL-21 in the other groups [2 months： high temperature group, （365.05 ± 18.17） ng/L, combined group, （278.66 ± 11.70） ng/L; 4 months： high temperature group, （343.73 ± 22.97） ng/L, ultraviolet group, （264.45 ± 23.96） ng/L, combined group, （150.90 ± 23.06） ng/L] were significantly lower than those in the control group [2 months： （518.11 ±18.68） ng/L; 4 months： （582.18 ± 28.90）ng/L] （P 〈 0.05）. The serum IL-21 levels of the 4-month ultraviolet group and the combined group were lower than the corresponding values of the 2-month groups （P〈0.01）. The level of serum IL-21 in the combined group was significantly lower than those of the high temperature group and the ultraviolet group treated for both 2 months and 4 months （P〈 0.05）. [ Conclusion ] The combination exposure of high temperature and ultraviolet may affect body weight gain, immune organ weight, and serum IL-21 levels of rats, and serum IL-21 levels would decrease with prolonged exposure time.
出处
《环境与职业医学》
CAS
CSCD
北大核心
2017年第8期712-717,共6页
Journal of Environmental and Occupational Medicine
基金
宁夏自然科学基金项目(编号:NZ15062)
关键词
紫外线
大鼠
高温
IL-21
免疫器官
ultraviolet
rat
high temperature
interleukin-21
immune organ
作者简介
郭金霞（1991-），女，硕士生；研究方向：职业与健康；E—mail：1097037480@qq．com
[通信作者]宋琦如，E-mail：sqr20@163．com
