摘要
利用SRAP和SSR标记对小白菜进行遗传多样性分析,从666对SRAP、160对SSR引物中筛选出59对多态性明显、条带清晰、稳定可靠的引物,对41份小白菜材料进行扩增,共扩增出519条带,平均每对引物扩增出6.2条,扩增出多态性条带数171条,多态性比例为32.9%。利用聚类软件进行分析,41份小白菜种间遗传距离在0.58~0.87之间,在遗传相似系数0.58处可将41份小白菜材料分为两大类。研究表明,小白菜品种具有丰富的遗传多样性,大多数小白菜种质资源之间的亲缘关系与其地理来源有较大的相关性。SRAP标记适用于分析种质的遗传距离,从种质资源保存的角度分析,SSR标记能够较全面地保证种质资源遗传多样性。
SRAP and SSR makers were used to analyze the genetic diversity of Chinese cabbage. 59 pairs of SRAP and SSR primers were selected from 666 SRAP and 160 SSR primers, which could produce stable and polymorphic bands. 519 bands were amplified from 41 samples and each primer resulted in 6.2 bands. 171 bands were polymorphic （ percentage of polymorphic bands was 32.9% ） . The value of Nei＇ s genetic variations indexes of 41 cuhivars based on the SRAP and SSR data with the cluster analysis varied from 0.58 to 0.87 and the cuhivars could be divided into two groups. The results showed that Chinese cabbage varieties owned abundant genetic diversities and most of them were clustered based on the source of cultivars. The investigation showed that SRAP was suitable for analyzing the genetic distance, while for conserving breeding resources, SSR would be a better choice to ensure genetic diversity. This study combined with SRAP and SSR markers for the first time to analyze the genetic diversity of Chinese cabbage, which would play an important role in Chinese cabbage breeding.
出处
《广东农业科学》
CAS
2017年第5期37-45,共9页
Guangdong Agricultural Sciences
基金
广东省科技计划项目(2014A020208042)
广州市科技计划项目(201508020004)
作者简介
李桂花（1975-），女，博士，副研究员，E—mail：398739253@qq．com
通讯作者：陈汉才（1962-），男，研究员，E-mail：13825086380@163．com
