摘要
应用重组聚合酶扩增技术(recombinase polymerase amplification,RPA),根据玛卡特异性基因defensin序列设计筛选了1对可用于扩增的引物,建立了玛卡及其加工产品特异性源性成分的RPA检测方法。该方法具有较高的灵敏性,检测灵敏度达到0.1 ng/L以上,操作方便,可以在室温条件下完成反应,检测可在0.5 h内完成,极大缩短了检测时间,适用于市场上玛卡及其制品的快速鉴别。
According to the defensin gene sequence of Lepich＇um meyenii, a pair of RPA primers was designed and a event-specific detection method for Lepich＇um meyenii and its derivates based on recombinase ploymerase amplification （RPA） was established. This method had high sensitivity with 0.1 ng/pL of DNA sensitivity, and was simple to operate. It could complete reaction at room temperature. The process could finish detection in 0.5 h. Detection time was greatly shortened. The RPA method was suitable for rapid identification ofLepidium meyenii and its derivates from the market.
出处
《粮食与油脂》
北大核心
2017年第7期51-54,共4页
Cereals & Oils
基金
广东省科自然科学基金项目(2016A030310102)
广东省质量技术监督局科技项目(2016CZ11)
关键词
等温扩增
玛卡
鉴别
Isothermal amplification
Lepidium meyenii
identification
作者简介
郭燕华（1984-），女，博士，工程师，研究方向为食品安全检测研究。
