摘要
目的比较外标法与相对校正因子法测定天山花楸黄酮含量的差别。方法采用高效液相色谱(HPLC)外标法测定天山花楸中金丝桃苷、芦丁、异槲皮素、槲皮素-3-O-(6″-O-丙二酰基)-β-D-葡萄糖苷、紫云英苷、山柰素-3-O-(6″-O-丙二酰基)-β-D-葡萄糖苷的含量;以金丝桃苷为内标物,采用HPLC相对校正因子法建立金丝桃苷与芦丁、异槲皮苷、槲皮素-3-O-(6″-O-丙二酰基)-β-D-葡萄糖苷、紫云英苷、山柰素-3-O-(6″-O-丙二酰基)-β-D-葡萄糖苷的相对校正因子,根据相对校正因子计算5种黄酮成分的含量,比较外标法与相对校正因子法的差异。结果 T检验外标法与相对校正因子法测定结果,差异无统计学意义(P>0.05)。结论外标法和相对校正因子法都可以用来测定天山花楸黄酮含量,但在缺少对照品或大批量检测的情况下,采用相对校正因子法测定天山花楸中的芦丁、金丝桃苷、异槲皮苷、槲皮素-3-O-(6″-O-丙二酰基)-β-D-葡萄糖苷、紫云英苷、山柰素-3-O-(6″-O-丙二酰基)-β-D-葡萄糖苷的含量快速可行,可作为新的质量评价模式用于天山花楸黄酮成分的质量评价。
Objective To compare the differences between external standard method and relative correction factor method for determination of the flavonoids from Sorbus tianschanica Rupr. Methods Using HPLC external standard method for determination of hyperoside,rutin,isoquercitrin,quercetin-3-O-（6″-O-malonyl）-β-D-glucoside,astragalin and Kaempferol-3-O-（6″-O-malonyl）-β-D-glucopyranoside in Sorbus tianschanica Rupr.,HPLC relative correction factor method was adopted to establish relative correction factor of the other five flavonoids above with hyperoside as reference.The difference was evaluated by comparing the external standard method with the relative correction factor method. Results There was no significant difference between the T test external standard method and relative correction factor method（P〉0.05）. Conclusion External standard method and relative correction factor method can be used for determination of the flavonoids from Sorbus tianschanica Rupr.,but in the case of lack of reference substance or mass detection,using the relative correction factor method for determination of rutin,hyperoside isoquercitrin,quercetin-3-O-（6″-O-malonyl）-β-D-glucoside,astragalin and kaempferol-3-O-（6″-O-malonyl）-β-D-glucopyranoside in Sorbus tianschanica Rupr. It was more feasible and it can be used as a new quality evaluation method in determination of flavonoid components from Sorbus tianschanica Rupr.
出处
《医药导报》
CAS
2017年第7期790-793,共4页
Herald of Medicine
基金
国家科技支疆计划项目(2008ZJ14)
关键词
天山花楸
金丝桃苷
芦丁
外标法
校正因子
相对
Sorbus tianschanica Rupr.
Hyperoside
Rutin
External standard method
Correction factor, relative
作者简介
冯荣（1991-）,女,新疆博尔塔拉蒙古自治州人,硕士,主要研究方向：天然产物分析.E-mail：409007049@qq.com.
通信作者,唐辉（1967-）,女,重庆人,教授,博士,主要研究方向：药物分析及新药研究.电话：0993-2057878,E-mail：th_pha@shzu.edu.cn.
