摘要
为了探讨GTP酶超基因家族中dynamin-1-like protein基因在昆虫中的表达特性,以松墨天牛为研究对象,从已构建的cDNA文库中筛选到松墨天牛dynamin-1-like protein基因,命名为Ma DLP1(Gen Bank:KU245763)。该序列长为2 133 bp,编码710个氨基酸。由此预测的蛋白质二级结构主要由α螺旋与无规则卷曲组成,其次是β片层与β转角;Ma DLP1基因编码蛋白质,定位于细胞核。通过DNAMAN软件比对发现Ma DLP1与赤拟谷盗的DLP1同源性最高,为82%,且存在3个保守酶域;用Clustal X和MEGA4.0构建系统发育树,显示松墨天牛与赤拟谷盗处在同一分支。RT-qPCR分析结果显示,Ma DLP1在各虫态不间断表达,幼虫期在5龄幼虫中表达量最高,化蛹期间表达量先上升后下降,羽化期间表达量表现为先上升后下降,并在初羽化的成虫中表达量达到最大值;Ma DLP1在幼虫和成虫头部表达量较高,且在幼虫脂肪体中表达最高;成虫的足、翅、触角和卵巢中也都有表达。说明Ma DLP1的表达与松墨天牛的完全变态发育相关。
To characterize the expression of dynamin-1-like protein gene of GTPase supergene family in in-sects, the gene named MaDLP1 （ GenBank： KU245763） was screened from the cDNA library of Monochamus al-ternatus. The sequence of MaDLP1 was 2133 bp in length, encoding 710 amino acids. The predicted secondary structure of MaDLP1 was mainly composed of alpha helix and random curl, followed by the beta turn and beta sheet. Subcellular localization of the gene was in nucleus. Amino acids sequence multiple alignment analysis of MaDLP1 showed the highest homology with Tribolium castaneum （ 82%） and there are three con-servative enzyme domains. Phylogenetic tree built by Clustal X and MEGA4 indicated M. alternatus with T. castaneum were in the same branch. RT-qPCR re-vealed that MaDLP1 expression may be associated with holometabolous development of M. alternatus; expression level peaked in the fifth instar larvae at larval phase, fell after rose in larval-pupal and eclosion phases, and reached the maximum amount in pupa-adult. The expression level was higher in the heads of larvae and adults, and was the highest in fat body.
出处
《江苏农业学报》
CSCD
北大核心
2017年第3期524-532,共9页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金项目(31470653)
广东省自然科学基金项目(2015A030313416)
作者简介
陈敬祥（1993-）,男,湖北荆州人,硕士研究生,研究方向为昆虫分子生物学.（Tel）13424049618;（E-mail）2441430459@qq.com
通讯作者：林同,（E-mail）lintong@scau.edu.cn
