摘要
目的:考察二黄凝胶贴膏的镇痛抗炎作用及其机制,为临床应用提供依据。方法:采用小鼠热板法、小鼠福尔马林致痛法与二甲苯致小鼠耳肿胀法、角叉菜胶致大鼠足肿胀法,贴敷给药7天,分别考察镇痛或抗炎作用,分别测定小鼠或大鼠血清中IL-1β及TNF-α的含量,探索镇痛或抗炎机制。结果:二黄凝胶贴膏4.57g/cm^2能显著减少福尔马林致痛小鼠舔足时间,二黄凝胶贴膏4.15g/cm^2能显著提高热板法小鼠痛阈值、减少福尔马林致痛小鼠舔足时间、降低小鼠血清中TNF-α含量,对IL-1β无影响,二黄凝胶贴膏4.57g/cm^2能显著减小角叉菜胶致大鼠足肿胀度、降低大鼠血清中IL-1β、TNF-α含量。结论:二黄凝胶贴膏具有明显的镇痛、抗炎作用,可能通过影响血清中IL-1β与TNF-α含量来实现。
Objective： To observe the analgesic, anti-inflammatory effects and its mechanism of Erhuang gel paste and to provide proof of clinic application. Methods： Hot plate test, formalin pain model, ear swelling induced by xylene in mice, and foot swelling induced by carrageen in rats were used. Erhuang gel paste was administered for seven days. Contents of IL-1β and TNF-α in serum of mice or rats were determined. Results ： 4.4g/kg Ehuang gel paste decreased the licking feet time of mouse in formalin pain model. 4.0g/kg Ehuang gel paste increased the pain threshold of mice in the hot plate test and decreased the licking feet time of mice in formalin pain model, decreased the content of TNF- α in serum. Meanwhile, 4.4g/kg Ehuang gel paste cured the foot swelling and decreased contents of IL-1β and TNF-α in serum in the carra- geen model. Conclusion： Erhuang gel paste has obvious analgesic and anti-inflammatory effects and it may be related to affecting the IL-1 β and TNF-α contents in serum.
出处
《中药药理与临床》
CSCD
北大核心
2017年第2期148-151,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家科技部科研院所技术开发研究专项(2012EG145135)
四川省科技支撑计划项目(2011SZ0306)
四川省骨科医院课题(2015-24)
关键词
凝胶贴膏
镇痛作用
抗炎作用
机制
Erhuang gel paste（凝胶贴膏）
analgesic effect
anti-inflammatory effect
