摘要
目的:研究青蒿琥酯(ART)对增生性瘢痕来源的成纤维细胞(HFBs)的Ⅲ型胶原(COLⅢ)蛋白表达的影响。方法:原代培养人HFBs,分为空白对照组和ART干预组(分别用75μmol/L、150μmol/L、300μmol/L、600μmol/L ART干预24h),荧光定量PCR(q-PCR)法检测Ⅲ型前胶原α1链(proCOL3A1)的基因转录情况,western blotting法检测COLⅢ蛋白的表达,酶联免疫吸附试验(ELISA)检测基质金属蛋白酶1(MMP-1)的含量。结果:与空白对照组比较,ART干预组的proCOL3A1 mRNA相对表达量明显降低,且不同浓度ART组间比较差异有统计学意义(P<0.01)。ART干预组COLⅢ蛋白表达水平明显下降,细胞培养上清液中MMP-1含量明显升高,且各浓度组间两两比较差异均有统计学意义(均P<0.05或P<0.01)。结论:ART能抑制人HFBs的COLⅢ的基因转录和蛋白水平,同时促进MMP-1分泌增加,加速胶原降解,抑制细胞外基质沉积,这可能是ART治疗增生性瘢痕的机制之一。
Objective： To investigate the effect of artesunate （ART） on the expression of type Ⅲ collagen （COL Ⅲ） in hypertrophic scar fibroblasts （HFBs）. Methods： HFBs isolated from tissue specimens of hypertrophic scar were treated with different concentrations （75, 150, 300 and 600 μmol/L） of ART for 24 h. The mRNA expression of proCOL3A1 was detected by fluorescent quantitative PCR （q-PCR）. The protein expression of COL Ⅲ was detected by western blotting. The matrix metalloproteinase （MMP） 1 level in supernatants was assessed by enzyme-linked immuno-sorbent assay （ELISA）. Results： The pro- COL3A1 mRNA level and the COL Ⅲ protein expression level were decreased, while the MMP-1 level in supernatants of culture medium was increased in ART groups as compared with the control group （ P 〈 0.01）. There were statistical differences in above indexes among different ART concentration groups （ P 〈0.05 or P 〈0.01）. Conclusion： ART may have a curative effect on hypertrophic scar, and the mechanisms may be related to the inhibition of MMP-1 release and the regulation of COL Ⅲ expression.
出处
《广西医科大学学报》
CAS
2017年第5期695-697,共3页
Journal of Guangxi Medical University
基金
国家自然科学基金资助项目(No.81060156)
广西自然科学基金资助项目(No.桂科青0447033)
广西科学研究与技术开发计划资助项目(No.桂科能14123006-2)
广西中医药科技专项重点课题(No.GZKZ10-010)
作者简介
通信作者，E-mail：xnong@gxmu．edu．cn
