摘要
建立一种快速、有效的高效液相色谱法测定运动饮料中双酚A残留的方法。样品经乙醇提取后,采用C18固相萃取小柱进行净化和富集。采用苯基色谱柱(4.6 mm×250 mm,5μm)分离,以乙腈∶水=40∶60(体积比)作为流动相,用PDA检测器检测,外标法峰面积定量。结果表明,双酚A标液在0.10μg/m L^1.00μg/m L浓度范围内线性良好,相关系数r^2为0.999,检出限为5.0μg/kg,定量限为15.0μg/kg,加标回收率达到92.1%~93.8%。该方法具有前处理简单、富集效果好和检测速度快的优点,适用于运动饮料中双酚A残留的定量检测。
A rapid and effective method was established for the determination of BPA in sports drink by HPLC.After the sample was extracted by ethanol,it concentrated and purified by C18 solid phase extraction column.The separation of targeted compound was performed on phenyl column（4.6 mm×250 mm,5 μm）using acetonitrile ∶ water（40 ∶ 60,volume ratio）as mobile phase,with PDA detector and external standard method peak area quantification. The linear range of BPA was in the range of 0.10 μg/m L-1.00 μg/m L with a correlation coefficient of 0.999. The detection limit was 5.0 μg/kg and the quantitative limit was 15.0 μg/kg.The recovery rate was92.1 %-93.8 %. The method has the advantages of simple pretreatment,good purifying effect and fast detection rate,and is suitable for the separation and quantitative detection of BPA in sports drink.
作者
韩明均
覃荣周
程敏
王琪林
HAN Ming-jun QIN Rong-zhou CHENG Min WANG Qi-lin(Aba Normal University, Wenchuan 623001, Sichuan, China Chengdu Sport University, Chengdu 610041, Sichuan, China)
出处
《食品研究与开发》
CAS
北大核心
2017年第8期149-152,共4页
Food Research and Development
关键词
高效液相色谱
运动饮料
双酚A
high performance liquid chromatography（HPLC）
sports drink
BPA
作者简介
韩明均（1982-），男（汉），讲师，硕士，研究方向：民族传统体育与体育保健康复。
