摘要
目的观察肺腺癌中CD147、MMP-9和VEGF蛋白表达,并分析三者表达的相关性。方法采用免疫组化SP法检测90例肺腺癌、10例肺良性病变和10例相对正常肺组织中CD147、MMP-9和VEGF蛋白表达,用Imagepro Plus图像分析软件测试3种蛋白在肺腺癌中的阳性强度。结果 CD147、MMP-9和VEGF蛋白表达的阳性单位(positive unit,PU)值在肺腺癌组织中均显著高于相对正常肺组织和肺良性病变组织(P均<0.001)。在90例肺腺癌中,CD147、MMP-9和VEGF的阳性率分别为88.89%(80/90)、84.44%(76/90)、80%(72/90)。CD147、MMP-9和VEGF均分别与肺腺癌组织学分化程度、淋巴结转移及TNM分期有关(P均<0.001),三者与肺腺癌患者年龄、吸烟史、大体类型均无关(P均>0.05)。CD147表达和MMP-9、VEGF表达均呈显著正相关(r_p=0.992,r_p=0.984,P均<0.001)。结论 CD147、MMP-9和VEGF可能在肺腺癌发生、发展中起重要作用,推测CD147刺激肿瘤细胞产生MMP-9和VEGF,进而促进肺腺癌的浸润和转移,可以作为肺腺癌免疫治疗的潜在靶点。
Purpose To investigate the expression of CD147, MMP-9 and VEGF and their relationships in lung adenocarcinoma. Methods The expression of CD147, MMP-9 and VEGF proteins was examined in 90 lung adenocarcinoma tissues by SP immunohistochemical method. The positive unit （PU） of CD147, MMP-9 and VEGF was assessed quantitatively using Imagepro Plus image analysis software. Results Positive intensity of CD147, MMP-9 and VEGF in lung adenocarcinoma was significantly higher than that in non-neoplastic internal controls and benign control tissues （P 〈 0. 001 ）. The positive rates of CD147, MMP-9 and VEGF were 88.89% （80/90） , 84.44% （76/90） , and 80% （72/90） , respectively. The expression of CD147, MMP-9 and VEGF was positively correlated with the de- gree of histologic differentiation, lymph node metastasis status and TNM stage （ P 〈 0. 001 ）. CD147 was correlated positively with MMP-9 and VEGF expression （ rp = 0. 992, rp = 0. 984, P 〈 0. 001 ）. Conclusion CD147, MMP-9 and VEGF may play an important role in the pathogenesis and progression of lung adenocarcinoma; CD147 may induce tumor invasion and metastasis by stimulating production of MMP-9 and VEGF, which is expected to be a potential promising target for immunotherapy.
出处
《临床与实验病理学杂志》
CSCD
北大核心
2017年第4期384-387,共4页
Chinese Journal of Clinical and Experimental Pathology
基金
郑州市普通科技攻关计划(20150076)
作者简介
徐小艳，女，硕士，主治医师。Tel：（0371）67077128，E—mail：xxy123corn@sina．com
杨金花，女，主任医师，通讯作者。Tel：（0371）67077350，E—mail：jinhuay65@sina．com
