摘要
目的通过对重症监护室（ICU）耐碳青霉烯类铜绿假单胞菌的分离率及同源性进行统计分析，评价重症监护室目前院内感染控制的状况。方法采用回顾性分析的方法，对2013年至2015年重症监护室患者的铜绿假单胞菌的分离结果进行对比性分析；采用肠杆菌基因间重复共有序列PCR（ERIC-PCR）和琼脂糖凝胶电泳的方法，对ICU3年间临床分离的耐碳青霉烯类铜绿假单胞的同源性进行检测分析。结果3年间铜绿假单胞的检出率差异无统计学意义（P〉0．05），耐碳青霉烯类铜绿假单胞检出率虽差异无统计学意义（P〉0．05），但数值有明显上升趋势。112株耐碳青霉烯类铜绿假单胞菌经基因分型，主要分为A、B、C、D、E5型，其中A型66株，B型34株，C型8株，D、E型各2株。结论ICU耐碳青霉烯类铜绿假单胞菌检出率呈上升的趋势，并呈高度同源性，可能存在科室内的克隆传播，应该合理化使用抗药物，以减少此类耐药菌的产生，并采取更有效的措施预防和控制其在院内的传播。
Objective To evaluate the current situation of nosocomial infection control in ICU through the analysis of the detach rate and homology of carbapenem-resistant Pseudomonas aeruginosa which from ICU in our hospital. Methods Contrastively analyzed detach outcome of Pseudomonas aeruginosa of ICU from 2013 to 2015 by retrospective analysis.Homology was analyzed by ERIC-PCR and agarose gelelectrophoresis. Results There was no statistical difference in the detach rate of Pseudomonas aeruginosa in three years （P〉0.05 ） .Although there was no statistical difference in the detach rate of carbapenem-resistant Pseudomonas aeruginosa.Its number was increased.The genotypes of 112 carbapenem-resistant Pseudomonas aeruginosa were analyzed by ERIC-PCR. Five genotypes could be obtained, A, B, C, D and E, 66 isolates were type A, 34 isolates were type B, 8 isolates were type C, type D and E each of 2 isolates. Conclusion The detach rate of arbapenem-resistant Pseudomonas aeruginosa of ICU shows an upward trend, and there has a high geneotyp homology within these isolates. These strains are likely to be clonally spread in the department. Antimicrobial agents should be used rationally to reduce the emergence of such resistant bacteria, and we should take more effective measures to prevent and control the spread of it in the hospital.
出处
《浙江临床医学》
2017年第5期957-958,共2页
Zhejiang Clinical Medical Journal
基金
浙江省自然科学基金项目（LY15H190006）
