摘要
This study was conducted to establish an analytical method for endosulfan residue in apple. Samples were extracted with 30 ml of acetone ： ethyl acetate （9：1）, purified with FlorisiL （containing activated carbon）, and detected by GC. Under those detection conditions, the detection limits of α-endosulfan, 15-endosulfan and endosulfate were 10^-11, 10^-11, and 2.5×10^-11 g, respectively. The recoveries of α-en- dosulfan were in the range of 89.49%-95.34%, with CVs in the range of 1.45%- 2.69%; the recoveries of β-endosulfan were in the range of 99.35%-100.63%, with CVs in the range of 1.39%-4.72%; and the recoveries of endosulfan suffate were in the range of 95.32%-101.15%, with CVs in the range of 1.19%-3.21%. The accu- racy, precision and sensitivity of the method all satisfy the requirements of pesticide residue analysis.
建立了硫丹(Endosulfan)在苹果中残留分析方法。样品用30 ml丙酮:乙酸乙酯(9:1)提取,5 g 5%水脱活处理的Florisi L(含活性炭)柱层析净化,气相色谱测定。硫丹3种单体的最小检测量分别为10^(-11)、10^(-11)、2.5×10^(-11)g。苹果样品中α-硫丹的添加回收率为86.54%~95.34%,变异系数为1.45%~4.73%;β-硫丹的添加回收率为82.91%~100.63%,变异系数为1.39%~4.72%;硫丹硫酸酯82.22%~101.15%,变异系数为0.75%~3.99%。方法的最低检出浓度分别为2.5×10^(-3)mg/kg,2.5×10^(-3)mg/kg,6.25×10^(-3)mg/kg。该方法的准确度、精确度、灵敏度均达到农药残留分析的要求。
基金
Supported by National Key Research and Development Program of China(2016YFD0200205-2)~~
作者简介
潘丹丹（1980-），女，安徽蒙城人，博士．讲师，主要从事环境污染物的毒理与生态研究，E-majl：dandanpan@ahau．edu．cn。E-mail：yueyd@icbr．ac.cn.
