摘要
以高山红景天为试材,采用IMAGING-PAM调制叶绿素荧光成像系统,测定了转UDP-葡萄糖:类黄酮3-O-糖基转移酶基因FaGT1的高山红景天及转FaGT1的RNAi作用基因FaGT1i的高山红景天的叶绿素荧光参数,分析转UDP-葡萄糖:类黄酮3-O-糖基转移酶基因的高山红景天叶片的叶绿素荧光特性,为研究红景天种质改良提供科学依据。结果表明:当光照强度为55μmol·m^(-2)·s^(-1)时,转FaGT1基因高山红景天的光系统Ⅱ的最大量子产量与高山红景天对照组和转FaGT1i基因高山红景天无显著差异,光系统Ⅱ潜在活性、非光化学淬灭系数/光化学淬灭系数和实际量子产量显著高于高山红景天对照组和转FaGT1i的高山红景天,但是调节性能量耗散的量子产量、非调节性能量耗散的量子产量则相对较低。另外,通过在不同的光照强度下,对光系统Ⅱ电子传递速率、光化学淬灭系数和非光化学淬灭系数进行了分析,得出转FaGT1基因高山红景天的光系统Ⅱ电子传递速率、非光化学淬灭系数呈先上升后稳定的趋势,显著高于高山红景天对照组和转FaGT1i的高山红景天;但是光化学淬灭系数呈先下降后平稳的变化趋势,与对照及转FaGT1i基因高山红景天无显著差异。转FaGT1基因高山红景天的光系统Ⅱ原初光能转换效率和潜在的光合活力均增强,植物自身的能量代谢得到提高,该研究结果为高山红景天的新种质培育提供了科学依据。
Rhodiola sachalinensis was used as test material.Modulated chlorophyll fluorescence system（IMAGING-PAM M-Series）was used to measure chlorophyll fluorescence parameters of Rhodiolasachalinensis transformed by UDP-glucose：flavonoid 3-O-glucosyltransferase gene to compare the chlorophyll fluorescence characteristics difference of Rhodiolasachalinensisand provided the scientific basis for germplasm improvement.The results showed that when exposed to photosynthetic active radiation（PAR）of 55-2μmol·m·s^-1,the FaGT1 gene transgenic Rhodiola sachalinensis,the FaGT1 igene transgenic Rhodiola sachalinensis and control had no significant difference in the value of maximum photosystem II quantum yield,the potential activity of photosystem II,non-photochemical quenching,photochemical quenching and effective quantum yield were significant higher in the FaGT1 gene transgenic Rhodiola sachalinensis than in the FaGT1 igene transgenic Rhodiola sachalinensis and control,but the regulated non-photochemical energy dissipation and non-regulated energy dissipation were lower.In addition,the differences of the electron transport rate,photochemical quenching and effective quantum yield in the FaGT1 gene transgenic Rhodiola sachalinensis,the FaGT1 igene transgenic Rhodiola sachalinensis and control under different PAR conditions.The electron transport rate and effective quantum yield were after the first increase in the stability of the trend and that significant higher in the FaGT1 gene transgenic Rhodiola sachalinensis than the FaGT1 igene transgenic Rhodiola sachalinensis and control,but the photochemical quenching was after the first decline in a smooth trend and had no significant difference.The primary light energy conversion of photosystemⅡand the potential photosynthetic activity had increased of the FaGT1 gene transgenic Rhodiola sachalinensis.The energy metabolism of plant itself has been improved.The results above provided scientific basis for the cultivation of Rhodiola sachalinensis germplasm.
出处
《北方园艺》
CAS
北大核心
2017年第6期108-112,共5页
Northern Horticulture
基金
吉林省科技发展计划资助项目(20150622022JC)
作者简介
未晓巍（1986-），女，吉林长春人，硕士，助理实验师，研究方向为植物基因工程。E—mail：weixiaowei@jlnu．edu．cn．
责任作者：周晓馥（1964-），女，博士，教授，博士生导师，研究方向为植物基因工程。E-mail：zhouxiaofu@jlnu．edu．cn．
