摘要
建立了高效液相色谱法测定草地早熟禾中玉米素（ZT）、吲哚乙酸（IAA）、脱落酸（ABA）和赤霉素（GA3）的方法。样品用80%甲醇水溶液冷浸提取。色谱柱为Extend-C_8（4.6×150mm,5μm）色谱柱,流速为1mL·min-1,进样量均为20μL。前3种激素的流动相为甲醇和0.6%冰乙酸梯度洗脱;检测波长为254nm。GA3流动相为乙腈：0.6%冰乙酸=60︰40等度洗脱;检测波长为208nm。4种激素在一定检测范围内具有良好的线性关系,相关系数均达0.99以上;平均回收率为83.78%～99.09%;相对标准偏差均小于5%。结果表明：该方法灵敏度、精密度和准确度好,可以满足草地早熟禾ZT,IAA,ABA和GA34种激素的检测。
A method has been developed for the detection of the contents of zeatin（ZT）,indoleacetic acid（IAA）,abscisic acid（ABA）and gibberellins（GAs）in Poa pratensis L.by HPLC（High Performance Liquid Chromatography）.After being extracted with 80% methanol from samples,four endogenous hormones were separated on a Extend-C8column（150×4.6mm,5μm）at flow velocity of 1mL·min-1 and injection volume of 20μL.ZT,IAA and ABA were detected at wavelength of 254 nm using methanol and0.6% acetic acid as mobile phase with gradient elution,While GA3 were detected at wavelength of 208 nm using acetonitrile and 0.6% acetic acid（60∶40）as mobile phase.There were good linear correlations between the concentrations and peak areas of the four hormones above the correlation coefficients of 0.99.The average recoveries were around 83.78%~99.09%,and relative standard deviations were below 5%.The results suggested that the method can be used to detect the contents of ZT,IAA,ABA and GA3 in Poa pratensis L.with good sensitivity,precision and accuracy.
作者
张咏梅
马晖玲
张锁科
唐云智
ZHANG Yong mei MA Hui-ling ZHANG Suo-ke TANG Yun-zhi(Instrumental Research and Analysis Center, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China Pratacultural college, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China)
出处
《草地学报》
CAS
CSCD
北大核心
2016年第6期1384-1387,共4页
Acta Agrestia Sinica
基金
国家自然科学基金(31160482)资助
关键词
草地早熟禾
内源激素
高效液相色谱
Poa pratensis L.
Endogenous hormone
High performance liquid chromatography
作者简介
张咏梅（1974-）,女,博士,副研究员,研究方向为草业科学与植物生理学,E-mail：zym824@sina.com
通讯作者： 马晖玲 E-mail： mahl@gsau.edu.cn
