摘要
为了探讨硒对新西兰兔毛囊发育的影响机制,本研究将60只初始体质量为(690.0±7.0)g的新西兰兔分为C和SS 2组,每组30只(雌雄各半)。C组直接饲喂基础日粮,SS组日粮添加亚硒酸钠,经测定日粮硒含量分别为0.20 mg/kg和0.36 mg/kg,饲养30 d。结果表明,C组新西兰兔的毛囊群结构排列整齐,1个初级毛囊周围环绕数个次级毛囊,且毛囊横切面呈正常椭圆形。SS组新西兰兔的毛囊群结构呈无序排列,初级毛囊和部分次级毛囊的横切面不是正常的椭圆形。SS组次级毛囊数量比C组低36.64%(P<0.05)。在皮肤抗氧化性方面,与C组相比,SS组皮肤组织中谷胱甘肽过氧化物酶4(GPx4)活性、超氧化物歧化酶(SOD)活性、总抗氧化物(T-AOC)含量分别降低58.09%(P<0.01)、34.39%(P<0.05)和59.25%(P<0.01),丙二醛(MDA)含量升高242.66%(P<0.05)。与C组相比,SS组新西兰兔皮肤毛囊中GPx4蛋白、表皮生长因子受体(EGFR)蛋白表达量分别降低131.70%(P<0.01)和128.60%(P<0.01)。
To investigate the effect of selenium on hair follicle development and expression of GPx4 protein in rabbit hair,60 New Zealand rabbits which initial weight was( 690.0±7. 0) g were divided into two groups( C and SS),30 rabbits in each group( male and female). The C group was fed on basal diet,the determination of dietary selenium level was 0. 20 mg / kg,SS group of dietary sodium selenite,the determination of dietary selenium level was 0. 36 mg / kg. Feeding 30 d.Rabbit hair follicle group structure of C group arranged neatly,a primary hair follicle was surrounded by a number of secondary follicles,hair follicles and oval cross section was normal. New Zealand rabbit hair follicle group structure of SS group was disordered,transverse section of pri-mary follicles and part of secondary follicles were not normal. The number of secondary follicles in SS group was decreased by 36. 64% than that in C group( P<0. 05). The antioxidant properties of the skin,compared with the C group,the GPx4 activity of SS group in skin tissue significantly decreased by 58. 09%( P< 0. 01),SOD activity significantly decreased by34. 39%( P<0. 05),T-AOC activity significantly decreased by 59. 25%( P<0. 01),the content of MDA significantly increased by 242. 66%( P<0. 05). The expression of GPx4 and EGFR proteins of New Zealand rabbit skin hair follicles in SS group were significantly decreased by the 131. 70%( P<0. 01) and 128. 60%( P<0. 01) than that in C group.
出处
《江苏农业学报》
CSCD
北大核心
2017年第1期133-140,共8页
Jiangsu Journal of Agricultural Sciences
基金
江苏省农业自主创新基金项目[CX(14)2078]
作者简介
杨杰（1972-）,男,安徽蒙城人,硕士,副研究员,主要从事家兔饲料营养调控研究.（E-mail）jieyangbwx@hotmail.com
