摘要
目的探讨电针对脑缺血再灌注大鼠皮质细胞凋亡相关蛋白Bcl-2、Bax及其mRNA表达的影响。方法雄性清洁级SD大鼠72只,采用随机数字表法分为假手术组、模型组和电针组各24只。用改良Longa线栓法制作大鼠右侧大脑中动脉脑缺血模型,缺血2 h,于再灌注开始后电针组针刺"百会"和"大椎"穴。各组于缺血再灌注24 h后行神经行为学评分和脑含水量测定,免疫组化染色法检测缺损侧皮层组织Bcl-2、Bax的蛋白表达,qRT-PCR检测Bcl-2 mRNA、Bax mRNA表达的变化。结果模型组、电针组神经行为学评分均低于假手术组(P<0.01),与模型组比较,电针组的神经行为学评分升高(P<0.05)。与假手术组比较,模型组脑含水量明显增高(P<0.01),电针组的差异无统计学意义(P>0.05),较之模型组,电针组的脑含水量显蓍降低(P<0.01)。与假手术组比较,模型组、电针组Bcl-2蛋白及mRNA的表达均显著增多(P<0.05或P<0.01),电针组又高于模型组(P<0.05);较之假手术组,模型组Bax蛋白及mRNA表达显著上升(P<0.01),电针组无明显差异(P>0.05),电针组较模型组显著降低(P<0.01);Bcl-2/Bax及Bcl-2 mRNA/Baxm RNA的比值,模型组降低而电针组升高(P<0.01)。结论电针可以通过提升Bcl-2/Bax的比值使抗凋亡基因占据优势,从而抑制缺血再灌注区的细胞凋亡,减轻脑水肿,促进神经功能恢复。
Objective To observe the influence of electroacupuncture（EA） on the expression of apoptosis related protein Bcl-2,Bax and mRNA in rats with cerebral ischemia and reperfusion. Methods The male 72 grade clean SD rats, using the random number table meth- od ,were divided into sham operation, model and EA groups with 24 rats in each group. The right middle cerebral artery occlusion models were induced by modified Longa suture method, and 2 hours later, EA group after repeffusion was given acupuncture ＂ Baihui＂ and ＂ Dazhui＂. The neurological behavior score and brain water content were measured by 24 hours after ischemia and reperfusion. The immunohis- tochemical staining method was used to detect defects of lateral cortex tissue Bcl-2, Bax protein expression, qRT-PCR was used to detect the changes of Bax and Bcl-2 mRNA expressions. Results The scores of neurological behavior in EA and model groups were lower than those in the sham operation group（P〈0. 01 ）;Compared With that of model group, the neurological behavior score of EA group was increased （ P〈0.05 ）. Compared with that of sham operation group, the brain water content was significantly increased in model group （ P〈0.01 ） ; How- ever, there was no significant difference in EA group （ P〉0.05 ） ; Compared with that of model group, the brain water content of EA group was decreased noticeable（P〈0.01 ）. Compared with those of sham operation and model groups, the expressions of Bcl-2 protein and mRNA of EA group were significantly increased（ P〈0.05 or P〈0.01 ）, the expressions of Bax protein and mRNA Bax of model group were significantly increased（P〈0.01 ）. The ratio of Bcl-2 mRNA/Bax mRNA and Bcl-2/Bax of model group were decreased and those of EA group were in- creased（P〈0.01 ）. Conclusions EA could improve the ratio of Bcl-2/Bax to take advantage of the anti apoptotic gene and to inhibit the Cell apoptosis, reduce the brain edema and promote the recovery of neural function.
出处
《中国老年学杂志》
CAS
北大核心
2017年第5期1041-1043,共3页
Chinese Journal of Gerontology
基金
国家自然科学基金资助项目(81373748)
作者简介
第一作者：范茜茜（1991-），女，硕士，主要从事电针对脑缺血再灌注损伤神经保护作用的凋亡机制研究。
通讯作者：董勤（1961-），女，教授，硕士生导师，主要从事针灸临床研究。
通讯作者：沈梅红（1970-），女，硕士生导师，主要从事针灸实验研究。
