摘要
目的:研究麝香、乳香配伍处理对前列腺干细胞增殖分化的影响。方法:选用7~10天龄的C57BL/6雄性幼鼠和16~18天龄的孕鼠,分别分离培养出前列腺上皮细胞和泌尿生殖窦间质(UGSM)细胞,并将二者混悬液移植到SCIDCB.17雄性小鼠的肾包膜下,30 d后收获移植物。将SCIDCB.17小鼠随机分为4组:麝香组、乳香组、麝香+乳香组、空白对照组,每组8只,按组别分别予麝香、乳香、麝香+乳香、生理盐水连续灌胃14 d后,取肾脏组织。通过HE染色形态学观察麝香配伍乳香对前列腺干细胞移植后腺管分化形成、各亚群干细胞分化状态影响,免疫组化和Western印迹检测前列腺干细胞抗原标志物P63、CD133、CD117、sca-1的表达分布情况。结果:建立了前列腺干细胞Lin-Sca-1^+CD49f^+(LSC)细胞及小鼠胚胎UGSM细胞的分离、混合培养体系;免疫组化结果示类前列腺腺泡上皮样结构P63、CD133、Sca-1、CD117染色呈阳性,证实移植物中可见前列腺腺泡上皮结构,且乳香组、麝香组的表达较空白对照组均有不同程度的增高,其中麝香+乳香组CD133、Sca-1、CD117免疫组化的积分光密度(IOD)值均显著高于单用乳香组和空白对照组(均<0.05),同时Sca-1的表达水平也显著高于单用麝香组(P<0.05),而P63的表达水平4组间无显著差异。Western印迹结果也显示,麝香+乳香组CD133、Sca-1、CD117的相对表达量均显著高于单用乳香组和空白对照组(P<0.05或<0.01),Sca-1的表达水平也显著高于单用麝香组(P<0.01)。结论:麝香、乳香配伍使用能促进前列腺干细胞的增殖再生。
Objective： To study the effects of musk-olibanum combination on the proliferation and differentiation of prostate stem cells. Methods： We cultured prostate epithelial cells and urogenital sinus mesenchymal（ UGSM） cells from 7- 10 d old C57 BL / 6 mice and 16- 18 d old pregnant C57 BL /6 mice,transplanted the mixed suspension of the two types of cells under the kidney envelope of SCIDCB. 17 male mice,and harvested the transplants 30 days later. We randomly divided the SCIDCB. 17 mice into four groups to be treated intragastrically with musk（ n = 8）,olibanum（ n = 8）,musk ＋ olibanum（ n = 7）,and normal saline（ blank control,n = 8）） respectively,all for 14 days. Then we collected the kidney tissue for observation of the morphology of the glandular tubes and differentiation of different subsets of stem cells by HE staining and determination of the expressions and distribution of P63,CD133,CD117 and Sca-1 by immunohistochemistry and Western blot. Results： A system was successfully established for the isolation and mixed culture of Sca-1 Lin-＋CD49f＋（ LSC） cells of prostate stem cells and UGSM cells of the mouse embryonic prostate. Immunohistochemistry showed positive expressions of P63,CD133,Sca-1,and CD117 in the prostatic acinar epithelia and proved the presence of prostatic acinar epithelial structure in the transplants. Compared with the blank control group,the expressions of CD133,Sca-1 and CD117 were significantly increased in the musk,olibanum,and musk ＋ olibanum groups（ P〈0. 05）,higher in the musk ＋ olibanum than in the musk or olibanum group（ P〈0. 05）,and their protein expressions were even more elevated in the musk ＋ olibanum group（ P〈0. 01）,with statistically significant difference from the olibanum group（ P〈0. 05）. Conclusion： The combination of musk and olibanum can improve the proliferation and differentiation of prostate stem cells. Natl J Androl,2017,23（ 2） ： 157- 163
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2017年第2期157-163,共7页
National Journal of Andrology
基金
国家自然科学基金资助项目(81573988)
湖南省高层次卫生人才"225"工程资助项目(湘卫人发[2013]13号)
湖南省科技计划重点研发项目(2015JC3075)
国家中医药管理局中医男科重点学科资助~~
作者简介
林群芳（1988-），女，江西上饶市人，硕士研究生，从事中医男科专业。
黄培（1990-），男，河南开封市人，硕士研究生，从事中医男科专业。
周青，Email：supergoon@163．COm
