摘要
面对能源危机,利用微藻生产生物燃料及其相关高附加值产品引起了人们的极大关注。微藻种类多样,繁殖速度快,不占用耕地,具有很高的油脂产量的潜能,是下一代食品、种子、化妆品和生物可再生能源最有希望的可选择性原料。发展这种资源的主要挑战是通过测定微藻中的无色油脂含量筛选富油品系,而与传统质量法检测油脂含量相比,荧光染料法更简单便宜、容易操作。主要介绍了尼罗红染料和BODIPY染料的特性,以及它们在微藻油脂测定中的应用和存在的问题。为快速准确检测微藻细胞的油脂含量,高效筛选优良产油藻株提供参考建议。
In the face of energy crisis,using microalgae to produce biofuel and high value-added products has attracted great attention.Microalgae constitute a diverse group of microorganisms with advantages like fast and efficient growth. In addition,they do not compete for arable land and offer very high lipid yield potential. Microalgae are currently considered as the most promising alternative sources for the next generation of food,feed,cosmetics and renewable energy in the form of biofuel. Major challenges for the development of this resource are to select lipid-rich strains using high-throughput staining for neutral lipid content in microalgae species. Compared with traditional quality method,the fluorescent staining method is simpler,cheaper and easier to operate. In this article,the properties of fluorescent Nile red and BODIPY and the use of fluorescent for lipid measurement in microalgae were summarized.
作者
郝翠翠
梁成伟
石蕾
HAO Cui-cui LIANG Cheng-wei SHI Lei(College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, Chin)
出处
《生物学杂志》
CAS
CSCD
2017年第1期70-74,共5页
Journal of Biology
基金
国家自然科学基金(31000135)
作者简介
郝翠翠，硕士，研究方向为植物基因工程，E—mail：291538667@qq．com
通信作者：梁成伟，副教授，研究方向为藻类生物学，E-mail：liangchw117@126．com
