摘要
目的观察富氢水对放射性皮肤损伤的作用。方法利用Varian直线加速器发射6Me V电子线照射大鼠后臀部皮肤制作损伤模型,治疗组予富氢水喷雾创面,对照组予生理盐水、持续4周,对比创面愈合情况,计算创面愈合率;MDA(丙二醛)、SOD(超氧化物歧化酶)试剂盒测定创面组织MDA含量和SOD活力,ELISA法测定血清IL-6和EGF含量。结果单次照射44Gy的6Me V电子线,成功建立动物模型。治疗后与生理盐水组相比,富氢水组创面愈合明显增快,创面组织MDA含量低、SOD活力高;血清IL-6含量较低、EGF含量高。结论富氢水能够促进放射性皮肤损伤的愈合,其作用机制与氧化损伤和炎性反应有关。
Objective To study the effect of hydrogen - rich water on skin radiation injury. Methods The skin radiation injury models on rats were established by locally irradiating with electron linear accelerator. Experimental group was applicated of hydrogen - rich water spray and control group was applicated of NS against rays after the skin ulcer for 4 weeks. The wound healing time was observed and wound healing rate were calculated. MDA content and SOD activity in wound tissue and IL -6 and EGF content in the blood were assessed by MDA, SOD assay kit and ELISA assay. Results The model of acute radiation - induced skin ulcers in rats was established with 44 Gy electron linear to the skin. The wound healing time of the hydrogen - rich water group was significantly shorter than the NS group. In the hydrogen- rich water group, the MDA content and IL- 6 content were lower, and SOD activity and EGF levels were significantly higher than the NS group after 1,2,3,4 weeks after administration. Conclusion Hydrogen - rich water can relieve oxidative damage and inflam- mation, which may be related to the positive effect of hydrogen - rich water in skin radiation injury.
出处
《医学研究杂志》
2017年第1期71-73,83,共4页
Journal of Medical Research
基金
海南省医药卫生科技项目(琼卫2013资助-091号)
海南医学院附属医院培育基金资助项目(HYFYPY201402)
关键词
氢
放射性损伤
皮肤
动物模型
Hydrogen
Radiation injury
Skin
Animal model
作者简介
通讯作者：王顺，硕士生导师，电子信箱：wangshun1963@163．com
