摘要
为了探索仔猪猪瘟免疫抗体的消长规律,制定科学有效的免疫方案,本试验采用目前较为常用的酶联免疫吸附法(ELISA法)和正向间接血凝法(IHA法),对仔猪出生后猪瘟母源抗体的水平进行跟踪检测,系统研究了猪瘟母源抗体消长规律,明确了仔猪进行初免的最佳时间。结果表明,母源抗体对初生仔猪有良好的保护作用,且在4~10d时抗体水平较高,保护率达到95%。但猪瘟抗体水平和群体合格率随着仔猪日龄的增加而下降,0~25d下降速度较为缓慢,32d时抗体水平和群体合格率显著降低(P<0.05)。虽然抗体水平超过临界保护值,但是群体合格率仅为40%,达不到农业部要求的合格率70%的标准,因此在25~32d进行首免较好。酶联免疫吸附法(ELISA法)和正向间接血凝法(IHA法)两种检测方法均具有很好的特异性,适用于日常的猪群免疫抗体水平的检测。
fn order to explore the dynamic changes of piglets swine fever immune antibodies and develop scientific and effective immunization scheme, enzyme-linked immune absorbent assay approach （ELISA） and indirect hemagglutination approach （IHA）, the more commonly used approaches at present, were adopted in the present experiments, which aimed to track and detect swine fever maternal antibody of postnatal piglets, study dynamic changes of swine fever maternal antibody in a system- atic manner, and determine the best time for primary immunization on piglets. The results showed that maternal antibody was capable to protect the newborn piglets effectively; the protection rate was 95% during a period from the 4th day to the 10th day. As increase of piglets age-in-day, the herd yield rate and antibody level decreased~ from 0 to the 25th day, the decline rate was slower, and on the 32nd day both the herd yield rate and antibody level decreased significantly（P〈0.05） . Although the antibody level exceeded the threshold protection value, the herd yield rate was 40% only, and less than 70%, the index as required by Ministry of Agriculture. Therefore, the primary immunization should be performed during a period from the 25th day to the 32nd day. The positive rate under the two approaches were Chi-square-tested, and it was shown from the results that there no significant difference between such two approaches（P〉0.05）. Both approaches were satisfied in specificity, and could be applied into detection of routine herd immunity antibody level.
出处
《畜牧兽医杂志》
2017年第1期9-11,共3页
Journal of Animal Science and Veterinary Medicine
作者简介
李曼（1986-），女，陕西安康人，硕士，兽医师，主要从事动物防疫和疫病诊断工作。E—mail：181704109@qq．com。
