摘要
成都平原位于长江流域带上游,是我国西南地区最主要的桃产业区域之一,对该地区的桃主栽品种进行遗传多样性分析并构建特异的分子指纹图谱对该地区桃种质资源和栽培应用具有重要的指导意义。该研究随机选择100对二核苷酸重复的NCBI-EST-SSR引物对40份桃主栽品种进行PCR扩增;然后选出12对多态性好、稳定性高的单位点扩增引物对材料进行遗传多样性分析。结果显示,40个品种的基因多样性指数在种级水平和品种群水平分别为0.579 6和0.428 3,遗传多样性高于先前的相关研究。在品种群水平上,直枝桃品种群的基因多样性指数最高(H=0.547 2),硬肉桃品种群次之(H=0.5417)。最后,采用"三步法"筛选得到6对EST-SSR引物组成区分效力最强的引物组合(pp079+pp098+pp074+pp016+pp075+pp063),并采用"+/-"矩阵为40份桃主栽品种构建了独一无二的EST-SST指纹图谱。该研究不仅为成都平原的桃主栽品种鉴定提供了分子依据,也为指纹图谱的实践运用补充了新的方向。
Chengdu plain,where is located in the upstream of yangtze basin,is one of the most important peach industry region in southwest of China.Therefore,It has an important guiding significance to analysis the genetic diversity of the peach main cultivars in this area and to construct series of unique molecular fingerprints for the peach germplasm resources and their employment of cultivation.PCR amplification was performed on the 40 peach main cultivars based on the 100 pairs of NCBI-EST-SSR primers that were randomly selected from dinucleotide repeat primers.Then,12 pairs of single-site amplified primers with high polymorphism and stability are chose to analysis the genetic diversity of the materials.The result showed that the Nei's expected heterozygosity of the 40 cultivars was higher than the related report previously with the value of 0.579 6 and 0.428 3 in species level and cultivar group level respectively.On the species group level,'ZZT'cultivar group possessed the highest Nei`s expected heterozygosity of 0.547 2,and the 'YRT'cultivar group followed with 0.541 7.Lastly,the primer group of the strongest distinguish potency which were consisted of 6 EST-SSR primers:pp079+pp098+pp074+pp016+ pp075+pp063 were screened by 'three steps'method,,and were used to construct series of unique fingerprints for the 40 peach mainly cultivars with '+/-' matrix.In this study,It not only provided a molecular reference for identifying the mainly cultivars in Chengdu plain,but also supplemented a new indication for practical use of fingerprints.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第3期638-646,共9页
Molecular Plant Breeding
基金
四川省教育厅重点项目(12ZZ011)
四川农业大学学科建设"双支计划"项目共同资助
