摘要
本研究应用SSR和SRAP 2种标记对11个苦瓜品种进行鉴定和亲缘关系分析。结果发现,应用筛选的8对SSR引物共扩增条带860条,多态性条带占68.0%,10对SRAP引物共扩增条带961条,多态性条带占79.4%。SSR标记检测到品种间遗传相似系数介于0.344~0.779,平均值为0.652;SRAP标记检测到品种间遗传相似系数介于0.373~0.700,平均值为0.625。聚类分结果发现,在遗传相似系数为0.54和0.52时,SSR和SRAP 2种标记都可以将11个品种分为相同的两组。每对SSR和SRAP引物能平均区分3.1和3.7个品种。最少用3对SSR引物或3对SRAP引物组合就可成功区分11个苦瓜品种。研究结果表明,SSR和SRAP标记均可高效地被用于苦瓜的品种鉴定和亲缘关系分析。
Abstract The study was carried out to identify and analyze the genetic relationship of 11 bitter gourd varieties(Momordica charantia L.) Results showed that 860 or 961 bands were amplified with screened 8 SSR pairs of primers or 10 SRAP pairs of primers, among which polymorphic bands account for 68.0% and 79.4%,respectively. The genetic coefficient threshold value displayed by SSR was from 0.344 to 0.779, the average equaled to 0.652. Accordingly, the threshold value displayed by SRAP was from 0.373 to 0.700, the average equaled to 0.625. The cluster analysis showed that 11 varieties of bitter guard were classified into 2 groups based on SSR and SRAP, and every pair of SSR and SRAP could identify 3.1 and 3.7 varieties respectively. All varieties could be identified successfully with at least 3 pairs of SSR or SRAP primers. All results indicated that both SSR and SRAP could be used for cultivar identification and genetic relationship analysis with high efficiency in Momordica charantia L.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第2期501-510,共10页
Molecular Plant Breeding
基金
广东省科技计划项目(2012A020602068)资助
