摘要
紫山药组培苗生根诱导培养基优化组合不同诱导试管生根苗的炼苗（移栽）、炼苗基质、季节、容器影响成活的比较试验。试验结果表明：紫山药试管生根苗以1/2 MS＋NAA mg/L＋AC g/L的生根率高,达标试管移栽苗3~5 cm长、茎粗、根系发达、叶片大深绿；基质以珍珠岩：黄土=3：1；营养钵容器在当年的4-5月份温度15~25℃,湿度控制在70%~80%下的弱光照或搭阴棚下炼苗较佳。炼苗成活率达70%~85%。
The optimum compositions of root inducing medium,various matrices for acclimation and transplantation,the effects of seasons and types of matrix container on the survival rate of test-tube plantlets of purple yam were tested. The results showed：the high root inducing rate was obtained in the medium of 1/2 MS supplemented with NAA mg/L＋AC g/L. The plantlets ,which reached to 3~5 cm height with strong stems,dark green leaves and well developed root systems,could be trans-planted ex vitro. During the period of April and May,the plantlets were transplanted into individual pot filled with pearlite：yellow earth （3：1） as matrix. The pots were then put in shady conditions with the temperature of 15~25℃ and the relative humidity of 70% ~80%,the acclimating survival rate could reach to 70% ~85%.
出处
《江西科学》
2016年第6期814-818,共5页
Jiangxi Science
基金
江西省科技厅支撑项目(20142BBF60034)
关键词
试管生根苗
培养基优化
基质容器筛选
成活率
test-tube plantlet
optimization of medium
matrix container
survival rate
作者简介
作者简介：王碧琴（1957-），女，研究员，主要从事植物遗传育种及生物技术研究工作。
通讯作者：余发新（1968-），男，博士，研究员，主要从事植物遗传育种研究工作。E-mail：fxyu2000@126．com。
