摘要
黄蜀葵花中富含黄酮类成分,具有保护心血管、改善肾功能等作用。该文采用3T3-L1前脂肪细胞诱导分化的成熟脂肪细胞,建立胰岛素抵抗模型,分为正常组,模型组,异槲皮苷(HY)、金丝桃苷(JY)、槲皮素(QT)、槲皮素-3'-O-葡萄糖苷(QG)、棉皮素-8-O-β-葡萄糖醛酸(GG)给药组,BCA法检测细胞培养液中葡萄糖的含量,荧光定量q PCR检测PPARγ,C/EBPα,SREBP-1,脂联素,内酯素,抵抗素基因mRNA的表达水平。结果显示,5种黄酮类化合物5μmol·L-1浓度时可促进3T3-L1前脂肪细胞的增殖,浓度为100μmol·L-1时可抑制前脂肪细胞的增殖;与正常组比较,模型组细胞对葡萄糖摄取量降低(P<0.01);与模型组相比,5种黄酮类化合物100μmol·L-1给药组细胞葡萄糖消耗量显著上升(P<0.01),PPARγ,C/EBPα,脂联素表达明显增加(P<0.01),SREBP-1,抵抗素,内酯素的表达明显降低(P<0.01),促使脂肪细胞分化。研究表明,HY,JY,QT,QG,GG能调控前脂肪细胞增殖,促进脂肪细胞分化及糖脂代谢相关因子PPARγ,C/EBPα,SREBP-1,脂联素,内酯素,抵抗素的表达,促进前脂肪细胞分化,增加葡萄糖利用,从而改善胰岛素抵抗。
Abelmoschus manihot was rich in flavonoids, which has been reported the activity on protecting angiocarpy and im- proving renal function. This study aimed to explore the action mechanism of five flavonoids from A. manihot on how to ameliora-ting insulin resistance through the regulation of the glucose and expression of PPARγ, C/EBPα, SREBP-1 , resistin, visfatin, adiponectin in 3T3-L1 adipocytes. After the 3T3-L1 preadipocytes were differentiated into mature adipocytes, insulin resistance model was built. Insulin resistance adipocytes were treated with 5, 100 μmol ·L^-1 quercetin, isoquercitrin, hyperoside, quer- citrin-3＇-O-glucoside, gossypetin-8-O-β-glucoside. The glucose was indirectly determined by BCA kit. The mRNA expression levels of PPARγ, C/EBPα, SREBP-1, resistin, visfatin, adiponectin were detected by real-time quantitative PCR. Results showed that five flavonoids at 5 μmol · L^-1 could accelerate preadipocytes proliferation and inhibit that at 100 μmol · L^-1 Com- pared with the normal group, glucose uptake reduced significantly in model group （ P 〈 0.01 ）. With the treatment of five fla- vonoids at 100 μmol · L^-1 , glucose consumption increased significantly （P 〈 0.01 ）. The high expression of PPART, C/EBPa, adiponectin expression was significantly increased （ P 〈 0.01 ） , and low expression of SREBP-1, resistin, visfatin after respec- tive administration with five flavonoids at 100 μmol · L^-1 promoted adipocyte differentiation. This study showed that, HY, JY, QT, QG, GG can control preadipocytes proliferation, promote adipocyte differentiation and regulate the expression of relative fac- tors with lipid metabolism, such as PPARγ, C/EBPα, SREBP-1, adiponectin, resistin, visfatin, increasing glucose utilization and improving insulin resistance in 3T3-L1 adipocyte.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2016年第24期4635-4641,共7页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81373889,81673533)
江苏省中药学优势学科Ⅱ期建设项目(ysxk-2014)
2013年国家教育部“新世纪优秀人才”计划项目(NCET-13-0873)
江苏省“333高层次人才培养工程”项目(BRA2015391)
作者简介
蔡红蝶，硕士研究生，E-mail：caihongdie@foxmail．com
[通信作者]宿树兰，教授，硕士生导师，主要从事中药资源化学及方剂功效物质基础研究，E-mail：sushulan1974@163．com；
[通信作者]段金廒，教授，博士生导师，E-mail：dja@njucm．edu．cn
