摘要
目的:研究槲皮素与5-氨基水杨酸(5-ASA)对感染后肠易激综合征(PI-IBS)的治疗作用及其机制。方法:PI-IBS大鼠模型分别灌胃给予5-ASA、槲皮素或5-ASA联合槲皮素,连续给药2周。腹壁收缩实验测定内脏痛阈值,水应激法评价结肠运动;收集血液和结肠,液质联用法测定5-ASA及其代谢物N-乙酰-5-ASA含量。结果:与正常组相比,PI-IBS组内脏痛阈值显著降低,结肠排便增多(P<0.05)。与模型组相比,5-ASA联合槲皮素可显著提高PI-IBS大鼠内脏痛阈值并明显减少结肠排便数量,其作用优于各单独用药组。槲皮素可明显增加PI-IBS大鼠结肠5-ASA含量并显著降低N-乙酰-5-ASA含量(P<0.05)。结论:槲皮素联合5-ASA对PI-IBS大鼠具有协同治疗作用,其机制可能与槲皮素抑制5-ASA代谢酶,提高结肠5-ASA浓度从而增强其治疗效果有关。
OBJECTIVE To investigate therapeutic effects and mechanism of quercetin and 5-aminosalicylic acid（5-ASA）on post-infectious irritable bowel syndrome（PI-IBS）in rats.METHODS PI-IBS rats were orally administered 5-ASA,quercetin or 5-ASA plus quercetin,respectively.Drugs were given to rats for 2 weeks consecutively.After treatment,visceral pain threshold pressure was measured by abdominal withdrawal reflex test,while colonic transit was evaluated by water avoidance stress test in PI-IBS rats.Then blood samples and colon tissues were collected for determination of concentrations of 5-ASA and its metabolite N-acetyl-5-ASA（N-AC-5-ASA）by LC-MS/MS method.RESULTS Compared to normal rats,PI-IBS rats presented significantly reduced visceral pain threshold pressure and increased fecal pellet output（P〈0.05）.Compared to PIIBS model rats,pain threshold pressure was markedly increased,while fecal pellet output was significantly reduced in rats treated with 5-ASA in combination with quercetin,and this therapeutic effect was superior to that of rats treated with 5-ASA or quercetin only.Moreover,quercetin treatment significantly increased content of 5-ASA but markedly reduced content of N-AC-5-ASA in colon tissues of PI-IBS rats（P〈0.05）.CONCLUSION Synergistic therapeutic effects are observed in PI-IBS rats treated with 5-ASA in combination with quercetin,and underlying mechanism may be correlated with inhibitory effects of quercetin on5-ASA metabolic enzyme,which increases colonic concentration of 5-ASA,and thus improves therapeutic effects of 5-ASA in PI-IBS rats.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2016年第23期2054-2058,共5页
Chinese Journal of Hospital Pharmacy
基金
国家自然科学青年基金项目(81400596)
甘肃省卫生行业科研计划管理基金项目(GWGL2013-25)
关键词
感染后肠易激综合征
槲皮素
5-氨基水杨酸
内脏痛敏
post-infectious irritable bowel syndrome
quercetin
5-aminosalicylic acid
visceral hyperalgesia
作者简介
张玉春，男，主管中药师，研究方向：中药鉴定与药效，电话：0931—8356510，E-mail：Zyc9912@163．com
[通讯作者]秦红岩，女，博士，副主任药师，研究方向：肠易激综合征发病机制及其药物治疗，电话：0931-8356511，E-mail：candyqinhy@163．com
