摘要
目的评估通过腹主动脉狭窄、冠状动脉结扎及腹主动脉狭窄联合冠状动脉结扎3种方法制备大鼠心力衰竭模型的差异。方法将雄性SD大鼠60只随机分为4组:对照组、腹主动脉狭窄组、冠脉结扎组及腹主动脉狭窄联合冠状动脉结扎组;各组大鼠在术后4周进行心脏超声观察射血分数(EF)、左室短轴缩短率(FS)、左室舒张(LVEDD)和收缩末期内径(LVESD)、左室舒张末期容积(LVEDV)和收缩末期容积(LVESV);左心室插管观测大鼠心率(HR)、左室收缩末压(LVESP)、左室舒张末压(LVEDP)及左室内压最大上升/下降速率(±dp/dtm ax),测量大鼠心脏质量,计算心脏质量/体质量比。结果对照组和腹主动脉狭窄组4周存活率100%,冠状动脉结扎组4周存活率为86.67%,腹主动脉狭窄联合冠状动脉结扎组4周存活率为13.33%。与对照组相比,腹主动脉狭窄组的EF、FS均明显降低(P<0.05或P<0.01),LVESD、LVESV和LVEDV明显升高(P<0.05或P<0.01),LVEDD和其他心功能指标没有明显变化;冠状动脉结扎组的EF、FS、LVSP和±dp/dtm ax均明显下降,LVEDD、LVESD、LVEDV、LVESV和LVEDP明显升高(P<0.01);除LVEDD外,其余各项指标与腹主动脉狭窄组相比有明显差异(P<0.01);与对照组相比,腹主动脉狭窄组的心脏质量/体质量比没有明显变化,冠脉结扎组心脏质量/体质量比明显升高(P<0.01)。结论腹主动脉狭窄法的心衰模型4周时大鼠的EF值仍处于正常范围,心衰症状不明显;冠脉结扎4周制作的心衰模型其心衰程度更为严重,能较好地模拟心衰的临床变化。
AIM To establish and assess the rat models of heart failure using abdominal aortic stenosis, coronary artery ligation or abdominal aortic stenosis combined with coronary artery ligation. METHODS Rats were randomly divided into four groups： control, abdominal aortic stenosis （AAS）, coronary aor- tic ligation （CAL） group, and abdominal aortic stenosis combined with coronary aortic ligation （AAS plus CAL）. At the fourth week after modeling, echocardiography and hemodynamics were used to assess cardiac functions. After measurement of rat heart weight, heart weight/body weight ratio was calculated. RESULTS At the fourth week, survival was 100% in the control group and AAS group, 86.67% in the CAL group, and 13.33% in the AAS ＋ CAL group. Ejection fraction （EF） and fractional shortening （FS） were reduced （P〈0. 05, P 〈0.01）, and LVESD, LVESV and LVEDV were increased in AAS group （P 〈 0. 05, P 〈 0. 01 ）. No significant changes were found in other parameters. EF, FS, LVSP and ＋ dp/dtm^x were reduced（P 〈0. 01 ）, whereas LVEDD, LVESD, LVEDV, LVESV and LVEDP were all increased in the CAL group （ P 〈 0. 01 ）, with significant difference between the AAS group and the CAL group in all aforementioned parameters except LVEDD. The heart mass index was increased in the CAL group （P 〈0. 01） and was not significantly changed in the AAS group compared with the control group CONCLUSION EF value is in the normal range and symptoms of heart failure in the AAS group are inconspicuous. Heart failure 4 weeks after CAL is more severe and this model can better simulate the clinical changes of heart failure.
出处
《心脏杂志》
CAS
2016年第6期671-675,共5页
Chinese Heart Journal
基金
国家重大新药科技重大专项资金资助(2009ZX09103-671)
国家自然科学基金项目资助(81270402)
陕西省自然科学基础研究计划项目资助(2014JM2-8168)
关键词
心力衰竭
腹主动脉狭窄
冠状动脉结扎
大鼠
heart failure
abdominal aortic stenosis
coronary aortic ligation
rat
作者简介
李雪健，本科生Email：547215655@qq．com
张晓，本科生Email：2291465482@qq．com
车运输，本科生Email：184076058@qq．com
通讯作者：张淑苗，实验师，博士，主要从事心脏内分泌研究Email：topsniper@126．com
