摘要
目的 2次回归旋转组合设计优化复合酶解法提取锁阳总糖工艺。方法以锁阳总糖得率为指标,酶解比例、酶用量、酶解温度、酶解时间、溶液初始p H值为考察因素,筛选出主要因素,并采用2次旋转组合设计方法优化提取工艺条件。采用响应面分析法(RSM),评价各因素水平的单因素效应、互作效应对响应值的影响。结果复合酶解法提取锁阳总糖最佳工艺:复合酶用量1%(锁阳粉末),酶解初始p H值5.5,酶解温度55℃,酶解时间45 min,果胶酶和纤维素酶比例1∶1,在此条件下锁阳总糖得率为(17.54±0.35)%。结论复合酶法提取锁阳总糖是一种简单有效的辅助方法,2次旋转正交组合设计法预测性良好。
Objective To optimize the extraction processes used for enzymatic hydrolysis of total sugar from Cynomorii Herba （Cynomorium songaricum） by employed quadratic orthogonal rotation regression combination design. Methods Taking the extraction rate of total sugar as index, as well as enzyme ratio, enzyme concentration, extraction temperature, extraction time, and initial pH value as investigation factors to screen out main factors. The quadratic orthogonal rotation regression combination design was applied to optimize the process. And then, to employ response surface analysis method （RSM） for evaluating the impacts on the response values of the single factor effect and interaction effect of each factor level. Results The optimum conditions were as follows： enzyme concentration was 1% （wt% of Cynomorii Herba powder）, initial enzyme solution pH was 5.5, extraction temperature was 55℃, extraction time was 45 min, and pectinase and cellulose was 1 ： 1 proportion. The extraction yield of total sugar was （17.54 ± 0.35 ） %. The influence of response values of each factor presented the relationship of the parabolic surface with the opening downward. The results showed that the impact degree on total sugar yield of Cynomorii Herba were extraction temperature, extraction time, and enzyme ratio, respectively. Conclusion This method is a simple and effective method to increase the extraction yield of total sugar from Cynomorii Herba. Quadratic orthogonal rotation regression combination design has a favourable predictability.
出处
《中草药》
CAS
CSCD
北大核心
2016年第22期4003-4008,共6页
Chinese Traditional and Herbal Drugs
基金
国家科技支撑计划项目(2012BAI30B01)
关键词
锁阳
总糖
复合酶
二次旋转正交试验
酶解
Cynomorii Herba
total sugar
complex enzyme
quadratic orthogonal rotation regression combination design
enzymatichydrolysis
作者简介
崔婷婷（1990-），女，硕士研究生，研究方向为锁阳多糖物质基础研究。Tel：（0993）2055002E-mail：739101237@qq．com
陈文（1967-），男，教授，研究方向为新疆特种植物药新药研究与开发。Tel：（0993）2055002E-mail：chen-wen2000@126．com
