摘要
目的 观察重组人促红细胞生成素 （ rhEPO ）对 ALI / ARDS 大鼠模型肺部炎症及肺 -毛细血管通透性的干预。方法 24 只雌性 SD 大鼠随机分为正常对照组、 ALI / ARDS 组、 EPO 低剂量组和 EPO 高剂量组。以腹腔注射内毒素进行造模,不同剂量的 rhEPO 进行干预。末次干预后收集BALF 计数炎症细胞以及蛋白总量;计量肺湿/干重比; HE 染色观察肺组织病理变化。结果 与正常对照组比较, ALI / ARDS 组肺组织病理损害严重,肺湿/干重比增加, BALF 中白细胞总数、中性粒细胞、淋巴细胞以及蛋白总量均显著增加 （ P 值均 〈0.01 ）;与 ALI / ARDS 组比较, EPO 低剂量组和EPO 高剂量组肺组织病理损害明显缓解,肺湿/干重比下降, BALF 中白细胞总数、中性粒细胞、淋巴细胞以及蛋白总量均显著下降 （ P 值均 〈0.01 ）,且 EPO 高剂量组效果优于 EPO 低剂量组 （ P 〈0.05 或 P 〈0.01 ）。结论 rhEPO 可以明显改善 ALI / ARDS 大鼠肺组织炎症水平,降低肺 - 毛细血管通透性,有效抑制 ALI / ARDS 的发病进程。
Objective To observe the effects of recombinant human erythropoietin （rhEPO） on acute lung injury （ALI）/acute respiratory distress syndrome （ARDS） rats. Methods Twenty-four female SD rats were randomly divided into four groups ： normal control group, ALI/ARDS group, low-dose EPO group, high-dose EPO group. ALI/ARD$ animal models were built by intraperitoneal injection of endotoxin, and giving different doses of rhEPO to intervene, White blood cells, neutrophils, Iymphocytes and total protein in bronchoalveolar lavage fluid （BALF） were measured. HE staining was used to observe the pathological changes in lung tissue. And lung wet/dry ratio was analysed. Results Compared with the normal control group, lung tissue was damaged seriously, lung wet/dry ratio increased, the numbers of white blood cells, neutrophils, lymphocytes and total protein in the BALF were increased significantly in the ALI/ARDS group （all P 〈 0.01）. Compared with the ALI/ARDS group, lung tissue damage was lighter, lung wet/dry ratio decreased, the numbers of white blood cells, neutrophils, lymphocytes and total protein in the BALF were significantly decreased in the low-dose and high-dose EPO groups （all P 0.05）. And the efficacy of high-dose EPO group was better than that of low-dose EPO group. Conelusions rhEPO can obviously improve lung tissue inflammation in ALI/ARDS rats, reduce the pulmonary capillary permeability, restrain the pathogenesis of ALI/ARDS effectively.
出处
《国际呼吸杂志》
2016年第22期1723-1726,共4页
International Journal of Respiration
作者简介
通信作者：张方琪，Email：sixchapters@163．com
