摘要
目的建立HPLC波长切换法测定清肺抑火片中黄芩苷、栀子苷和芒果苷的含量。方法色谱柱为Waters Sun Fire C18（4.6 mm×150 mm,5μm）;流动相为甲醇（A）-0.1%磷酸水溶液（B）系统,梯度洗脱,流速为1.0 m L·min-1,柱温：30℃,波长切换时间序列采样：0-9 min为238 nm,9-20 min为258nm,20-35 min为280 nm。结果黄芩苷进样量在0.194-3.877μg与峰面积线性关系良好（r=0.9999,n=7）,平均回收率在98.4%-100.5%,RSD均〈3.0%（n=3）。栀子苷进样量在0.037-0.745μg与峰面积线性关系良好（r=0.9998,n=7）,平均回收率在99.4%-100.7%,RSD均〈3.0%（n=3）;芒果苷进样量在0.016-0.324μg与峰面积线性关系良好（r=0.9999,n=7）,平均回收率在99.6%-100.7%,RSD均〈3.0%（n=3）。结论该方法准确、快捷、可靠,可更好地用于清肺抑火片的质量控制。
Objective To establish an HPLC wavelength switching method to simultaneously determine baicalin, geniposide and mangiferin in Qingfei Yihuo tablets. Methods Waters Sun Fire C18（4.6 mm×150 mm, 5 μm） column was used. The detection wavelength was 238 nm（0-9 min） for geniposide, 258 nm（9-20 min） for mangiferin, and 280 nm（20-35 min） for baicalin. Results Baicalin had a good linearity at 0.194-3.877 μg, and the average recovery was 98.4%-100.5% with RSD〈 3.0%（n = 3）. Geniposide had a good linearity at 0.037-0.745 μg, and the average recovery was 99.4%-100.7% with RSD〈 3.0%（n = 3）. Mangiferin had a good linearity at 0.016-0.324 μg, and the average recovery was 99.6%-100.7% with RSD〈 3.0%（n = 3）. Conclusion The method is simple, accurate and reproducible. It can be used to determine geniposide, mangiferin and baicalin simultaneously in Qingfei Yihuo tablets with the same chromatogram condition.
作者
唐德智
TANG De-zhi(Nanning Institute for Food and Drug Control Nanning 530021)
出处
《中南药学》
CAS
2016年第11期1253-1256,共4页
Central South Pharmacy
关键词
清肺抑火片
黄芩苷
栀子苷
芒果苷
HPLC波长切换法
Qingfei Yihuo tablet
baicalin
geniposide
mangiferin
HPLC wavelength switching method
作者简介
唐德智，男，副主任中药师，主要从事中药质量标准控制研究，Tel：（0771）3132340，E-mail：tangdz7497@163．com
