摘要
目的采用多种表面技术复合在纯镁表面制备了功能性生物复合膜层,以调控医用纯镁降解速度,赋予纯镁内固定材料的抑菌性和生物活性。方法分别通过纯镁微弧氧化,电镀壳聚糖、海藻酸钠,化学镀铜等方法,制作不同复合膜层,分别为纯镁微弧氧化膜层(A组)、纯镁微弧氧化-壳聚糖复合膜层(B组)、纯镁微弧氧化-壳聚糖/海藻酸钠复合膜层(C组)、纯镁微弧氧化-壳聚糖/海藻酸钠/壳聚糖复合膜层(D组)和纯镁微弧氧化-壳聚糖/海藻酸钠载铜复合膜层(E组)。采用扫描电子显微镜分析膜层的微观形貌,测定润湿角,将材料与大肠杆菌共同培养,计算抑菌率。结果 A组表面有很多微孔和少量微裂纹,B组表面的微孔和微裂纹减少,C组的微孔和裂纹基本消失,D组和E组表面的微孔裂纹消失并出现微网状结构。A组和E组为亲水性膜层,C组为疏水性膜层,B组和D组的膜层接近疏水性。A组抑菌率最低,B、C、D组的逐渐增高,分别为26%、30%、61%,E组的抑菌率高达88%。结论纯镁微弧氧化-壳聚糖/海藻酸钠载铜复合膜层有封孔和愈合裂纹的作用,具有较好的抑菌性。
The work aims to reduce degradation speed of medical pure magnesium and endow fixation materials in medical pure magnesium with antibacterial activity and biological activity by preparing functional composite coatings on the surface of pure magnesium by combining a variety of surface technologies. Different composite coatings, including pure magnesium micro-arc oxidation（MAO） coating（group A）, pure magnesium MAO-chitosan composite coating（group B）, pure magnesium MAO-chitosan/alginate composite coating（group C）, pure magnesium MAO-chitosan/sodium alginate/chitosan composite coating（group D） and pure magnesium MAO-chitosan/alginate con-taining copper composite coating（group E）, were made by pure magnesium micro-arc oxidation, electroplating of chitosan and sodium alginate and chemical plating copper. Microstructures of the composite coatings were analyzed by scanning electron microscopy（SEM）. The wetting angles of the coatings were measured and the materials were co-cultured with Escherichia coli. After 24 hours, their bacteriostatic rates were calculated. The results indicated that there were many micropore and a few microcracks on the surface of group A, pores and microcracks on the surface of group B reduced, that on the surface of group C mostly disappeared while microcracks on the surface of group D and E totally disappeared and micro reticular structures appeared. The coatings in group A and E were hydrophilic; for group C, hydrophobic; for group B and group D, nearly hydrophobic. The antibacterial rate of group A was the lowest; that of group B, C and D, which was respectively 26%, 30% and 61%, increased gradually; that of group E was up to 88%. Pure magnesium MAO chitosan/sodium alginate containing copper composite coating can seal pores and heal cracks and is of good antibacterial activity.
出处
《表面技术》
EI
CAS
CSCD
北大核心
2016年第11期159-166,共8页
Surface Technology
基金
佳木斯大学研究生科技创新项目(LZZ2015_022)
国家自然科学基金(31370979)~~
关键词
纯镁
微弧氧化
壳聚糖
海藻酸钠
铜
抑菌性
pure magnesium
micro-arc oxidation
chitosan
alginate
copper
antibacterial activity
作者简介
刘继光（1961-），男，教授，主要从事生物医学材料的研究工作。
通讯作者：李慕勤（1955-），女，博士，教授，研究方向为生物医学材料表面改性。
