摘要
目的:探讨细胞骨架蛋白Ezrin和AnnexinⅡ在胆囊癌发展转移过程中的意义。方法:应用免疫组织化学(Max-Vision)技术对含有59例胆囊癌组织及6例正常胆囊组织的组织微陈列芯片进行Ezrin和AnnexinⅡ表达的检测;应用Western blot方法检测20例新鲜胆囊癌标本和6例正常胆囊标本中Ezrin和AnnexinⅡ的表达。并收集临床病理数据,与检测结果作统计分析。结果 :免疫组织化学检测结果表明:胆囊癌组织中Ezrin和AnnexinⅡ的阳性表达率分别为47.5%和50.8%,Ezrin和AnnexinⅡ在胆囊癌组织中的表达明显高于在正常胆囊组织中的表达,Ezrin的阳性表达与肝脏侵犯、淋巴结转移和Nevin分期有关;AnnexinⅡ的阳性表达与肝脏侵犯、分化程度和Nevin分期有关;胆囊癌中的Ezrin与AnnexinⅡ的表达有统计学相关性(r=0.423,P<0.05)。Western blot结果提示:Ezrin和AnnexinⅡ在胆囊癌组织中呈高表达,在正常胆囊组织中低表达,Ezrin和AnnexinⅡ的高表达与肝脏侵犯相关。结论:Ezrin和AnnexinⅡ可能是反映胆囊癌侵袭和转移潜力的重要生物学标记。检测Ezrin和AnnexinⅡ的表达对评估胆囊癌的恶性生物学行为有一定的临床意义。
Objective To investigate the expression levels of Ezrin and AnnexinⅡ in gallbladder carci-noma and their association with clinicopathologic parameters and metastasis potential. Methods The tissue mi-croarray consisted of 59 gallbladder carcinoma tissues and 6 normal gallbladder tissues were examined for the ex-pression of Ezrin and AnnexinⅡusing immunohistochemistry technique. The expression of Ezrin and AnnexinⅡin 20 cases of fresh gallbladder carcinoma and 6 cases of normal gallbladder were measured with western blot. Results The expression of Ezrin and AnnexinⅡ were higher in the gallbladder cancer than those in the normal gallbladder tissue. The positive rate of Ezrin and AnnexinⅡ were 47.5% and 50.8% respectively. The expression of Ezrin was significantly correlated with live metastasis , lymph node metastasis and Nevin stages. The expression of AnnexinⅡwas significantly correlated with live metastasis , differentiation levels and Nevin stages. The expres-sion of Ezrin was correlated with AnnexinⅡ. Results of western blot suggested that Ezrin and Annexin II were highly expressed in gallbladder carcinoma tissues. The high expression of Ezrin and Annexin is closely related with liver invasion. Conclusion Measurement of the expression of Annexin and Ezrin II have important clinical significances to evaluate the malignant biological behavior of gallbladder carcinoma.
出处
《实用医学杂志》
CAS
北大核心
2016年第19期3147-3150,共4页
The Journal of Practical Medicine
基金
江苏省卫生厅科研立项(编号:H201347)
作者简介
通信作者：瞿甦E-mail：chyg7676@163．com
