摘要
目的建立自动顶空-气相色谱(HS-GC)内标曲线法测定血中乙醇含量的不确定评估方法。方法从分析测定程序着手,依据不确定度评定的指导性文件,分析不确定度来源,量化不确定度分量,计算检测结果的合成标准不确定度和扩展不确定度。结果各相对不确定度来自于检材重复性检测为3.4%,乙醇标准溶液为0.71%,检材为0.61%,叔丁醇内标溶液为0.41%,标准曲线为1.1%,气相色谱仪为1.3%,血液中乙醇的相对扩展不确定度为3.9%。结论血液中乙醇含量的不确定度主要来源于检材重复性检测、气相色谱仪、乙醇标准曲线。
ObjectiveTo evaluate the uncertainty for the determination of ethanol in human blood by auto-headspace gas chromatography （HS-GC） with internal standard curve method.Methods Each source of uncertainty, arising from the procedure of testing, was analyzed and conifrmed according to the guidelines of the uncertainty in measurement . After each uncertainty component was quantized, the combined standard uncertainty and the expanded uncertainty of the result were calculated.Results The relative uncertainty brought from the measurement repeatability, standard solution of the ethanol, the sample of blood, internal standard solution of the tert-butyl alcohol, the calibration cure, gas chromatography were 3.4%,0.71%,0.61%,0.41%,1.1% and 1.3% respectively; the relative expanded uncertainty of ethanol in blood was 3.9%.Conclusion The measurement uncertainty of the concentration of ethanol was came primarily from the measurement repeatability of sample, HS-GC and standard curve of ethanol.
出处
《中国法医学杂志》
CSCD
2016年第4期364-366,共3页
Chinese Journal of Forensic Medicine
关键词
法医毒物分析
不确定度评估
自动顶空-气相色谱内标曲线法
乙醇
forensic toxicological analysis
measurement uncertainty
auto HS-GC internal standard curvemethod
ethanol
作者简介
梁丽军，女，工程师，硕士，主要从事毒品、毒物、微量物证的检验。E-mail：lianglj84@126．com
