摘要
目的 :观察右美托咪定预处理对心肌缺血再灌注损伤(myocardial ischemia reperfusion injury,MIRI)大鼠线粒体功能的影响,探讨其对缺血再灌注心肌的保护作用。方法:将30只健康雄性SD大鼠随机分为3组:假手术组(A),MIRI组(B),MIRI+右美托咪定组(C)。采用结扎冠脉左前降支的方法建立心肌缺血再灌注损伤大鼠模型,通过心脏超声检测大鼠心功能的变化,real-time PCR检测线粒体功能相关基因m RNA水平的表达。透射电镜观察心肌线粒体超微结构。结果:与A组相比,B组和C组心肌功能受损,线粒体功能相关基因m RNA表达均降低(P<0.05);与B组相比,C组心肌功能损伤减轻,线粒体功能相关基因m RNA表达水平明显增加(P<0.05)。与A组相比,B组线粒体超微结构损伤显著;与B组相比,C组线粒体损伤程度明显减轻。结论:右美托咪定预处理可以减轻缺血再灌注所致心肌线粒体功能损伤,改善心肌功能,发挥保护心肌作用。
Objective：To investigate the effects of dexmedetomidine preconditioning on mitochondrial function of myocardial ischemia reperfusion injury（MIRI）,and to explore the protective effect of dexmedetomidine preconditioning on myocardium with ischemia and reperfusion injure. Methods：A total of 30 healthy male Sprague Dawley（SD）rats,aged 8 weeks,were randomly divided into three groups：the sham operation group（group A）,the myocardial ischemia-reperfusion group（group B）,and the dexmedetomidine preconditioning group（group C）. The model of myocardial ischemia reperfusion injury was performed by ligation of the left anterior descending coronary artery. Cardiac functions were detected by echocardiography. The expression of the m RNA levels associated with mitochondrial function was performed by real-time PCR. Ultrastructure of myocardial mitochondria was observed under electronic microscope. Results：Compared with group A,the cardiac function of group B and group C were markedly decreased;the m RNA levels of genes related to mitochondrial function were significantly lower in group B and group C than that in group A（P〈0. 05）.Compared to group B,the cardiac function of group C was enhanced;the m RNA levels of genes related to mitochondrial function were higher in group C than in group B（P〈0.05）. We also found that impaired mitochondrial ultrastructure was observed in group B.Compared with group B,impaired mitochondrial structure was significantly improved in group C. Conclusion：Dexmedetomidine preconditioning could improve mitochondrial function to alleviate myocardial ischemia reperfusion injury.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第10期1198-1201,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
镇江市社会发展基金(Sh2014087)
关键词
右美托咪定
心肌缺血再灌注损伤
线粒体
dexmedetomidine
myocardial ischemia reperfusion injury
mitochondria
作者简介
通信作者（Corresponding author），E-mail：sdhjsdxfsrmyy@126．com
