摘要
目的研究瓜蒌薤白滴丸(GX)对大鼠心肌缺血再灌注损伤(MIRI)的保护作用。方法将经筛选合格的SPF级大鼠随机分为4组,每组12只,假手术组、模型组分别给予生理盐水,GX组和复方丹参滴丸组分别给予相当于原药22.5 g/kg和85.05 mg/kg的滴丸,1次/d,给药7 d,末次给药后1 h采用结扎大鼠冠状动脉30 min后,解结扎120 min进行MIRI造模。记录造模前后心电图的变化,测定血浆肌酸激酶MB(CK-MB)、心肌肌钙蛋白T(CTNT)、心肌肌红蛋白(MYO)含量,通过光镜、电镜观察心肌组织病理变化,采用伊文思蓝灌注染色法测定心肌梗死百分率。结果与模型组比较,GX可对抗MIRI过程中心电图ST段的抬高,明显降低大鼠血浆CK-MB、MYO、CTNT含量(P<0.05或P<0.01),改善心肌细胞纤维排列紊乱的状况,减轻心肌细胞组织间的水肿,减少肌纤维间的炎细胞浸润和心肌细胞变性坏死,降低心肌梗死百分率(P<0.01)。结论 GX对大鼠MIRI有保护作用。
Objective To verify the protective effects of Gualou Xiebai dropping pills（GX）on myocardial ischemia-reperfu-sion injury（MIRI）in rats. Methods After screening the qualified SPF rats were randomly divided into four groups,with 12 in each group. Sham-operated group and model group rats were respectively treated with normal saline,and rats in GX group and compound Danshen dropping pills group were given the corresponding dropping pills equivalent to 22.5 g/kg and 85.05 mg/kg of the crude herb,re-spectively. All groups were administered once a day for 7 successive days. One hour after the last administration,the MIRI models were produced by occluding the left coronary artery for 30 min and releasing the occlusion for 120 min. The changes in ST segment were observed,and the contents of creatine kinase-MB（CK-MB）,cardiac troponin-T（CTNT）and myoglobin（MYO）in blood plas-ma were measured. The pathological changes in myocardial tissues were observed by optics and electric-microscope and the percentage of myocardial infarction was measured by detecting the content of Evan blue in myocardium. Results Compared to normal saline in the model group,GX had antagonism to ECG S-T segments elevation in rats with myocardial ischemia,while the contents of CK-MB, MYO,and CTNT in blood plasma declined significantly（P〈0.05 or P〈0.01）;the disorder condition of myocardial cell fiber arrange-ment was improved,and edema between myocardial tissue and cells was relieved;the inflammatory cell infiltration and myocardial ne-crosis in cardiac allograft were significantly alleviated,and the percentage of myocardial infarction was decreased significantly（P〈0.01）. Conclusion GX may play an important protective role against the MIRI in rats.
出处
《国际药学研究杂志》
CAS
CSCD
北大核心
2016年第5期926-930,共5页
Journal of International Pharmaceutical Research
基金
安徽高校省级自然科学研究重大项目(KJ2015ZD41
KJ2016SD60)
安徽省科技攻关项目(12010402089)
国家大学生创新创业训练计划项目(201410368013)
皖南医学院重点培育基金项目(WK2014Z04)
关键词
瓜蒌薤白滴丸
心肌缺血再灌注损伤
保护作用
Gualou Xiebai dropping pills
myocardial ischemia-reperfusion injury
protective effects
作者简介
邹纯才，男，副教授，研究方向：药物制剂及其质量控制，E-mail：zouche@163．com
通讯作者：鄢海燕，女，教授，研究方向：药物制剂及其质量控制，Tel：0553-3932185，E-mail：yhy0801@126．com
