摘要
为快速生产大量优质白芨种苗,以白芨种子为外植体进行离体快繁研究。结果表明:MS基本培养基可促进白芨种子的快速无菌萌发,当6-BA的浓度为1.0 mg/L时可以促进幼苗的快速分裂生长,添加了2 mg/L的2,4-D的培养基可有效促进愈伤组织的形成。种子苗原球茎经切割后在MS+2 mg/L 2,4-D+0.5 mg/L6-BA的培养基上愈伤组织诱导率为85%,1.0 mg/L的6-BA和0.15 mg/L的NAA配比可以诱导丛生芽的生长和增殖,添加了70 g/L香蕉泥和0.5 mg/L NAA的1/2MS培养基可有效促进根的生长。无菌发芽的种子苗移栽率可以达90%,而试管苗移栽的成活率较低,但随离体培养时间的延长有所提高。
To propagate the high quality seedlings of Bletilla striata rapidly,the seeds were used as explants for in vitro culture. The results showed that,MS basic medium could promote the rapid aseptic germination of bletilla seeds. When the concentration of 6 — BA was 1. 0 mg / L could promote the rapid growth of seedlings. The medium added with 2 mg / L2,4 — D could effectively promote the formation of callus. The callus induction rate on the medium of MS ＋ 2 mg / L 2,4— D ＋ 0. 5 mg / L 6 — BA was 85%. 1. 0 mg / L 6 — BA and 0. 15 mg / L NAA could induce the rapid proliferation of clump buds. And the medium of 1 /2MS added with 0. 5 mg / L NAA and 70 g / L banana puree could effectively promote the root development. The transplant survival rate of aseptic seedling was 90%,whereas the survival rate of the plantlets was lower.
作者
张豆豆
彭晓英
朱永州
杨亚博
敖东阳
ZHANG Doudou PENG Xiaoying ZHU Yongzhou YANG Yabo AO DongyangI(Bioscience and Technology College , Hunan Agricultural University, Changsha 410128, China Longtanhe Agro-teehnical Station in Cili, Cili 427228, China)
出处
《湖南林业科技》
2016年第5期47-51,共5页
Hunan Forestry Science & Technology
基金
长沙市科技局一般项目(K1406021-21)
2014年度湖南农业大学生科院大学生创新性研究项目
关键词
白芨
愈伤组织
快速繁殖
增殖系数
Bletilla striata
callus
rapid propagation
proliferation coefficient
作者简介
张豆豆（1993-），女，河南省漯河市人，本科，研究方向：生物技术专业。
通讯作者：彭晓英，女，博士，副教授；E-mail：xypeng@hunau．net。
