摘要
背景:短期高强度间歇运动训练是否能改善心肌梗死后心脏功能及其相关作用机制尚不清楚。目的:观察高强度间歇运动训练对心肌梗死后心脏功能及线粒体含量的影响,并探讨线粒体自噬和生物合成在其中的生物效应。方法:选取雄性SD大鼠制备急性心肌梗死模型,术后1周进行高强度间歇运动训练,训练方案为:以80%最大摄氧量快速跑4 min,间歇以40%最大摄氧量慢速跑3 min,重复7个循环,5 d/周,共4周。结果与结论:4周高强度间歇运动训练显著提高急性心肌梗死后左心室泵功能和线粒体含量,增加线粒体膜电位和ATP合成活力,抑制线粒体活性氧产生,上调PGC-1α/Tfam介导的线粒体生物合成和Bnip3/Beclin-1介导线粒体自噬。结果表明短期高强度间歇运动训练即可提高急性心肌梗死后心肌健康线粒体含量,从而改善心肌能量代谢和舒缩功能,是一种时效性较强的心脏康复手段。
BACKGROUND： It is unclear whether short-term high-intensity interval training（HIT） can be used to protect against myocardium injury after acute myocardial infarction, as well as the underlying mechanism. OBJECTIVE： To observe the effects of short-term HIT on the ventricular remodeling and mitochondrial content after acute myocardial infarction, and the biological effect of mitochondrial biogenesis and autophagy in this process. METHODS： Male Sprague-Dawley rats were modeled into acute myocardial infarction by ligating coronary artery. One week later, HIT was performed： each interval consisted of 4-minute high-intensity running（80% of maximal oxygen consumption） and 3-minute active recovery（40% of maximal oxygen consumption）, for 4 consecutive weeks of 5 days each week, repeated 7 cycles. RESULTS AND CONCLUSION： Four-week HIT after acute myocardial infarction could markedly enhance left ventricular pump function and mitochondrial content, improve mitochondrial membrane potential and ATP synthetic activity, inhibit mitochondrial reactive oxygen species generation, up-regulate PGC-1α/Tfam induced mitochondrial biogenesis and Bnip3/Beclin-1 induced autophagy. These results indicate that short-term HIT can improve normal mitochondrial content after acute myocardial infarction, which in turn ameliorates myocardial systolic property and energy metabolism. As a cardiac rehabilitation method, HIT exhibits fine timeliness.
出处
《中国组织工程研究》
CAS
北大核心
2016年第40期5986-5993,共8页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(31200894
31571224)
武警后勤学院博士启动基金(WHB201507)
军队2110工程三期建设项目共同资助~~
作者简介
何炜，男，1977年生，宁夏回族自治区银川市人，汉族，在读博士，主治医师，主要从事心血管医学研究。
通讯作者：薄海，博士，副教授，武警后勤学院天津市心血管重塑与靶器官损伤重点实验室，天津市300162；武警后勤学院军事训练医学教研室，天津市300309
