摘要
旨在探讨外源脱落酸(ABA)对低温胁迫下小桐子幼苗耐冷性及甜菜碱积累的影响。用150μmol/L ABA处理低温胁迫下的小桐子幼苗,研究其对小桐子幼苗存活率,根系活力,电解质渗漏率,MDA含量,甜菜碱含量及其代谢关键酶BADH活性和Jc BADH基因表达水平的影响。结果表明,外源150μmol/L ABA处理可显著提高低温胁迫下小桐子幼苗的存活率和根系活力,降低电解质渗漏率和MDA含量;ABA也提高了低温胁迫下小桐子幼苗的甜菜碱含量,上调了BADH的活性和Jc BADH的表达水平。因此,ABA可提高低温胁迫下小桐子幼苗的耐冷性,并且在ABA诱发的小桐子幼苗耐冷性的提高过程中,甜菜碱发挥着重要作用。
The objective is to investigate the effect of exogenous abscisic acid （ ABA ） on chilling tolerance and glycine betaine accumulation of Jatropha curcas seedlings under chilling stress. Seedlings of J. curcas under chilling stress were treated with 150 μmol/L ABA, and the survival rate, root vitality, electrolyte leakage, the content of malondialdehyde （ MDA ）, the content of glycine betaine, and the activity of the key1~enzyme betaine aldehyde dehydrogenase （ BADH ） of glycinebataine biosynthesis as well as the expression of JcBADH were measured. The results showed that treatment with 150 μmol/L ABA significantly increased survival rate and root vitality, decreased MDA content and electrolyte leakage in J. curcas seedlings under chilling stress. ABA also enhanced the accumulation of glycine betaine, increased the activity of BADH, and improved the expression level of the JcBADH. The results suggested that exogenous ABA treatment may enhance chilling tolerance in J. curcas seedlings, and glycine betaine might play a key role in the increasing of chilling tolerance.
出处
《生物技术通报》
CAS
CSCD
北大核心
2016年第9期100-106,共7页
Biotechnology Bulletin
基金
国家自然科学基金项目(31260169
31260064
31460059)
云南师范大学研究生科研创新基金项目(38)
关键词
脱落酸
低温胁迫
甜菜碱
耐冷性
小桐子
abscisic aC＇id
chillir/g stress
glycine betaine
chilling tolerance
Jatropha curcas
作者简介
杨双龙，男，博士，研究方向：植物逆境生物学，E-mail：yangs1318@163．com；
邓凤飞为本文并列第一作者
通讯作者：龚明，男，博士，研究方向：植物逆境生物学；E-mail：gongrning63@163．com
