摘要
目的：建立高效液相色谱法测定清平颗粒中人参皂苷Rg1含量。方法：色谱柱为Agilent ZORBX Eclipse plusC18（4．61mm×100mm，3．5μm），流动相：乙腈（A）-水（B），检测波长：203rim，流速：1．0mL／min，柱温：25℃，进样量：10μL。结果：人参皂苷Rg。在0．0132～0．4230mg／mL范围内呈良好线性关系，r=1．0000；回收率：98．0％，RSD：0.3％。结论：该测定方法操作简便，准确可行，可用于清平颗粒中人参皂苷Rg1的定量分析。
Objective： To establish a HPLC method for determination of ginsenoside Rg1 content in qingping keli. Methods： Separation was carried out on an Agilent ZORBX Eclipse plus C18（4.61mm×100mm,3.5μm）column using acetonitrile （A）-water （B） as mobile at a detection wavelength of 203 nm and a flow rate of 1.0 mL/min. The column temperature was 25℃ while the sample load was 10μL. Results： The linear range of ginsenoside Rg1 concentration was 0.013 2-0.423 0 mg/mL（r = 1.000 0）. The average recovery of sample was 98.0%, with a RSD of 0.3%. Conclusion： This method is simple, accurate and feasible, which may be used for the quantitative determination of ginsenoside Rg1 in qingping keli.
作者
石晶萍
洪丽萍
Shi Jingping Hong Liping(Yangtz River Pharmacitical Group Co., Ltd, Jiangsu Taizhou 225321, China)
出处
《中国执业药师》
CAS
2016年第9期23-27,共5页
China Licensed Pharmacist
作者简介
洪丽萍，女，执业药师。研究方向：药物分析和标准研究。通讯作者E-mail：hongliping@yangzijiang.com
