摘要
长链非编码RNA(Long non-coding RNA,lnc RNA)是一类长度大于200个核苷酸、缺少蛋白编码功能的RNA。lnc RNA可从多层面调控基因表达,影响表型性状。鸡作为重要经济动物和模式生物,lnc RNA研究相对滞后。为加快鸡lnc RNA研究进展,利用公共数据库(如NCBI-SRA等)中鸡高通量转录组测序(RNA-seq)数据,通过生物信息学方法发掘8 040条鸡lnc RNA,发现大量组织特异性表达lnc RNA,为鸡lnc RNA功能研究奠定基础。
Long non-coding RNAs （IncRNAs）, one class of functional RNAs longer than 200 nucleotides, lack of protein coding potential, are involved in a diverse array of biological processes. LncRNA can affect the expression of phenotype, by regulating gene expression in multiple ways. Besides its great economical value, chicken is widely used as an excellent animal model, however, chicken IncRNA research was still lagging behind. Here, we collected public RNA-Seq data sets of chickens from the NCBI-SRA database. Bioinformatics pipeline and methods were then established for the identification and tissue-specific expression analysis of IncRNAs in chickens. In total 8 040 transcripts were identified as putative IncRNAs, and chicken IncRNAs tend to have higher expression levels in neuronal tissues than other tissues. The findings lay the foundation for the further functional analysis of chicken IncRNAs.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2016年第7期40-47,共8页
Journal of Northeast Agricultural University
基金
国家863计划项目(2013AA102501)
现代农业产业技术体系建设专项资金资助项目(CARS-42)
国家自然科学基金(31472088)
作者简介
李辉（1963-），男，教授，博士，博士生导师，研究方向为动物遗传育种。E-mail：lihui@neau．edu．cn
