摘要
目的比较不同放射敏感性鼻咽癌患者血清蛋白质表达差异,筛选出与鼻咽癌放射抗拒性相关的蛋白质。方法提取不同放射敏感性鼻咽癌患者血清蛋白,应用核素标记相对和绝对定量(i TRAQ)技术标记蛋白,联合液相色谱和串联质谱(LC-MS/MS)分离、分析肽段。采用Proteome Discoverer 1.4软件对蛋白进行鉴定和定量分析,对获得的差异蛋白进行GO分析。结果共鉴定出差异表达的蛋白质65个,其中上调34个、下调31个,GO分析显示差异表达的蛋白质主要涉及生物学过程调控、压力应激反应及分解代谢等生物学过程。结论 S100A7、胰岛素样生长因子结合蛋白2、α1抗胰蛋白酶和热休克蛋白等差异表达的蛋白质可能与鼻咽癌放射抗拒性相关。
Objective To discover the proteins related to nasopharyngeal carcinoma（ NPC） radioresistance by screening differentially expressed proteins in NPC patients with different radiosensitivity. Methods Two groups of sera were respectively collected from NPC patients with different radiosensitivity（ 20 casesineach group）. Isobaric tags for relative and absolute quantitation（ iTRAQ）-taggingcombined with liquid chromatography tandem mass spectrometry（ LC-MS / MS） analysis was used to identification of differentially expressed proteins between the two groups. The identification and quantitation of the proteins were analyzed by Proteome Discoverer 1. 4 software. The proteins differentially expressed were analyzed by GO（ Gene Ontology） terms. Results A total of 65 differentially expressed proteins were identified,of which 34 were up-regulated and 31 were down-regulated. GO analysis showed these proteins to be involved in keybiological processes such as biological regulation,response to pressure stress and catabolism. Conclusion Analysis identify the following 4 proteins that may be the candidate biomarkers for predicting radioresistance of NPC： S100A7,insulin-like growth factor-binding protein 2,alpha-1-antitrypsin and HSP 70.
出处
《安徽医科大学学报》
CAS
北大核心
2016年第9期1333-1337,共5页
Acta Universitatis Medicinalis Anhui
基金
安徽省自然科学基金(编号:1408085MH189)
安徽省卫生厅医学科研课题(编号:13ZC009)
关键词
鼻咽癌
辐射抗拒
蛋白质组学
nasopharyngeal carcinoma
radioresistance
proteomics
作者简介
高劲，男，副教授，副主任医师，硕士生导师，责任作者，E—mail：gj11667@126．com
