摘要
目的:利用基因芯片技术研究RNA干扰下调PPP2R5C对K562细胞中TP53通路相关基因表达的影响。方法:Affymetrix U133 plus 2.0基因表达谱芯片检测核转染PPP2R5C-siRNA991和SC对照组的K562细胞,应用Genespring GX 11.0软件分析差异基因相关信号通路变化情况。结果:基因芯片分析TP53信号通路紧密相关的22个基因,发现全部基因发生了差异表达,其中上调基因有8个,下调基因有14个。明显上调的基因有EP300、CCND1、BRCA2和USP7,而明显下调的基因则有MDM4、ESR1、ATM、CDK2、CDKN1A、TP53和CDKN2A。结论:下调K562细胞PPP2R5C基因时,在一定程度上选择性调节与细胞增殖密切相关的TP53信号通路相关基因的表达,从而抑制K562细胞增殖,促进了K562细胞凋亡。
Objective：To investigate effect of down-regulated PPP2R5C expression on TP53 related pathway gene expression in K562 cells using gene chip technology with RNA interference. Methods：Affymetrix U133 plus 2.0 gene chip was used to determine PPP2R5C-siRNA991 nuclear transfected K562 cells and SC transfected K562 cells in the control group. Genespring GX 11.0 software was used to analyze the changes of the differential gene. related signaling pathway. Results：Twenty-two genes closely related to TP53 signaling pathway were analyzed by gene chips,and all genes were differentially expressed,including 8 up-regulated genes and 14 down-regulated genes. The significantly up-regulated genes were EP300,CCND1,BRCA2 and USP7. The significantly down-regulated genes were MDM4, ESR1, ATM, CDK2, CDKN1A, TP53 and CDKN2A. Conclusion： Down-regulation of PPP2R5C gene in K562 cells can selectively regulate TP53 related pathway gene expression,which is closely related to cell proliferation,thereby inhibiting the proliferation K562 cells and promoting the apoptosis of K562 cells.
出处
《广州医科大学学报》
2016年第3期1-4,共4页
Academic Journal of Guangzhou Medical University
基金
深圳市卫生计生系统科研项目(201505005)
深圳市科技计划项目(JCYJ20150403101028195)
广东省高等学校科技创新重点项目(cxzd1013)
广东省自然科学基金(S2013020012863)
广东省高等学校高层次人才资助项目(粤财教[2013]246号-54)
作者简介
通讯作者：Email：jnyangqiuli@163．com
